| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,683,278 | T→G | 13.2% | Y145S (TAT→TCT) | yhgF ← | predicted transcriptional accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,683,278 | 0 | T | G | 13.2% | 66.6 / 12.7 | 38 | Y145S (TAT→TCT) | yhgF | predicted transcriptional accessory protein |
| Reads supporting (aligned to +/- strand): ref base T (17/16); new base G (2/3); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
ATCAGGATATAGCGCGCGCCATCCAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGCCAACGGCTC > minE/2683212‑2683342 | aTCAGGATATAGCGCGCGCCATCCAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACata > 1:1838651/1‑68 (MQ=255) tCAGGATATAGCGCGCGCCATCCAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACat < 1:682619/66‑1 (MQ=255) atatAGCGCGCGCCATCCAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAgc < 1:1992037/71‑1 (MQ=255) taGCGCGCGCCATCCAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGCGCAgcggc < 1:1915487/71‑1 (MQ=255) cgcgcgcCATCCAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGAc < 1:77253/71‑1 (MQ=255) cAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCgtgt > 1:299290/1‑71 (MQ=255) cAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCgtg < 1:118044/70‑1 (MQ=255) cAGCGCGGCTTTGGTATCTG‑‑ACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCgtgtgt > 1:1589796/1‑71 (MQ=255) cgcgGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCgtgtgt < 1:556031/70‑1 (MQ=255) ggCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACg < 1:1239745/71‑1 (MQ=255) gCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATtgtga < 1:246588/44‑2 (MQ=255) gCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACgg < 1:574346/71‑1 (MQ=255) tGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATc > 1:1289139/1‑70 (MQ=255) tATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACg < 1:1461271/63‑1 (MQ=255) tATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACg < 1:1373054/63‑1 (MQ=255) tCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACgg < 1:2178940/62‑1 (MQ=255) tCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCca > 1:1698869/1‑71 (MQ=255) cTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTcc > 1:466551/1‑69 (MQ=255) tGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGAt > 1:623388/1‑62 (MQ=255) tGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCca > 1:1668230/1‑69 (MQ=255) tGCCACGCCTTTATCGGCATAAACAGATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCca > 1:1282266/1‑69 (MQ=255) gCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCAcag > 1:531462/1‑71 (MQ=255) cACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCAcagca < 1:1184183/71‑1 (MQ=255) cGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAgg < 1:514106/71‑1 (MQ=255) ccTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGt > 1:1840297/1‑70 (MQ=255) ccTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGt > 1:1940773/1‑70 (MQ=255) tttATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCgtgtgt < 1:993030/46‑1 (MQ=255) tttATCGGCATAAACAGATTGTGCAGCGGCGACTTCTGGCGTGTGTGACgg < 1:1613880/51‑1 (MQ=255) tttATCGGCATAAACAGATTGTGCAGCGGCGACTTCTGGCGTGTGTGACgg < 1:1857307/51‑1 (MQ=255) tttATCGGCATAAACAGATTGTGCAGCGGCGACTTCTGGCGTGTGTGACgg < 1:1753776/51‑1 (MQ=255) tATCGGCATAAACATATTGTGCAGCTGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGc > 1:1491683/1‑70 (MQ=255) tATCGGCATAAACAGATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGc > 1:713127/1‑70 (MQ=255) aTCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGcc > 1:593307/1‑70 (MQ=255) ggCATAAACATATTGTGCAGCGGCCACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGCTCAGCCAACg > 1:659635/1‑71 (MQ=255) tAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGGCAGCCAACGGCTc < 1:265892/71‑1 (MQ=255) aaaCATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGCCAACGGCTc < 1:1198074/70‑1 (MQ=255) aaCATATTGTGCAGCGGCGACTTCTGGCGTGTGTGa > 1:771346/1‑36 (MQ=255) aaCATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCca > 1:1806122/1‑48 (MQ=255) aaCATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCca > 1:1004702/1‑48 (MQ=255) aaCATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGCCAACGGCTc > 1:1588521/1‑69 (MQ=255) tatTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGCCAACGGCTc < 1:452108/65‑1 (MQ=255) tatTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGCCAACGGCTc < 1:1747720/65‑1 (MQ=255) | ATCAGGATATAGCGCGCGCCATCCAGCGCGGCTTTGGTATCTGCCACGCCTTTATCGGCATAAACATATTGTGCAGCGGCGACTTCTGGCGTGTGTGACGGATCGCTCCACAGCAGGTCAGCCAACGGCTC > minE/2683212‑2683342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |