| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,703,672 | G→A | 33.3% | A355T (GCC→ACC) | aroB → | 3‑dehydroquinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,703,672 | 0 | G | A | 33.3% | 70.1 / 50.1 | 57 | A355T (GCC→ACC) | aroB | 3‑dehydroquinate synthase |
| Reads supporting (aligned to +/- strand): ref base G (18/20); new base A (8/11); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 | |||||||||||
ATTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGC > minE/2703606‑2703739 | aTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCAt > 1:1010129/1‑71 (MQ=255)aTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCAt > 1:1079622/1‑71 (MQ=255)aTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCAt < 1:1395816/71‑1 (MQ=255) cttcCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGc > 1:1291147/1‑71 (MQ=255) cttcCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGc > 1:1167882/1‑71 (MQ=255) ccGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCg < 1:1158156/70‑1 (MQ=255) cAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCaa < 1:1538323/71‑1 (MQ=255) aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt < 1:694697/71‑1 (MQ=255) ggTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAg > 1:1454045/1‑70 (MQ=255) ggTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGc > 1:865339/1‑71 (MQ=255) agagTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTa < 1:1943158/70‑1 (MQ=255) agagTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaa > 1:766469/1‑71 (MQ=255) gagTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTaac < 1:1816978/71‑1 (MQ=255) gTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa > 1:1714029/1‑71 (MQ=255) gTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGATCTTAACGCCATTGCCGATTGTCaa < 1:1897727/59‑1 (MQ=255) tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGc < 1:2152325/42‑1 (MQ=255) tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGc < 1:1805253/42‑1 (MQ=255) tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATc > 1:1075578/1‑60 (MQ=255) tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATc > 1:1099415/1‑60 (MQ=255) gTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCTATTGTCAATCAGCGTAACAACAAGa < 1:475335/71‑1 (MQ=255) gTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGa < 1:1114196/71‑1 (MQ=255) ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAAcaacaa < 1:653270/68‑1 (MQ=255) ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGa < 1:854709/70‑1 (MQ=255) tCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaac > 1:1398266/1‑62 (MQ=255) cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAacaaca > 1:1873565/1‑65 (MQ=255) aGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTc < 1:916186/71‑1 (MQ=255) aGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGt > 1:1146193/1‑70 (MQ=255) gcggcgTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGt > 1:2018258/1‑69 (MQ=255) gcggcgTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGGCa < 1:1211077/71‑1 (MQ=255) gcgTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGc > 1:1556049/1‑71 (MQ=255) cgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGcc < 1:260424/71‑1 (MQ=255) tCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt > 1:1936661/1‑70 (MQ=255) tCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt > 1:430234/1‑70 (MQ=255) cGCACGAGCTTGTTCTTAACACCATTGCCTATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCtt < 1:1733340/70‑1 (MQ=255) cGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa > 1:442354/1‑51 (MQ=255) cGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCaa < 1:348672/71‑1 (MQ=255) gAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAg < 1:73579/71‑1 (MQ=255) gAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAg < 1:1800314/71‑1 (MQ=255) gCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCg < 1:273851/71‑1 (MQ=255) gTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCa < 1:1719619/63‑1 (MQ=255) gTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc > 1:1327563/1‑70 (MQ=255) gTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGt < 1:81594/69‑1 (MQ=255) gTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc < 1:1239074/70‑1 (MQ=255) gTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc < 1:1000676/70‑1 (MQ=255) gTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc < 1:1590160/70‑1 (MQ=255) ttCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGt < 1:326920/48‑1 (MQ=255) ttCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGt < 1:1917889/48‑1 (MQ=255) ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAgg < 1:1774502/47‑1 (MQ=255) cTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTa > 1:2191021/1‑69 (MQ=255) cTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTAt < 1:271293/70‑1 (MQ=255) cTTAACGACATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg > 1:151664/1‑49 (MQ=255) ttAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTa > 1:1603232/1‑56 (MQ=255) aaCGCCATTGCCTATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTAtt < 1:213269/68‑1 (MQ=255) aaCGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAg > 1:1931198/1‑41 (MQ=255) aaCGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAg > 1:917216/1‑41 (MQ=255) aaCGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGc > 1:328447/1‑71 (MQ=255) aaCGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGc > 1:1142225/1‑71 (MQ=255) | ATTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGC > minE/2703606‑2703739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |