| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,726,174 | G→A | 35.7% | R303R (CGC→CGT) | yheS ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,726,174 | 0 | G | A | 35.7% | 26.6 / 24.7 | 28 | R303R (CGC→CGT) | yheS | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (8/10); new base A (4/6); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
ATGCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTC > minE/2726106‑2726233 | atGCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGcgcgcg > 1:1045304/1‑71 (MQ=255)atGCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGcgcacg > 1:1548554/1‑71 (MQ=255) gATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGATTTCCGGCGCACGGAAg > 1:781348/1‑71 (MQ=255) gCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACTGATTTGGCAGGCTTTCCGGCGCACGGAAGCTa < 1:1148361/70‑1 (MQ=255) gCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTa < 1:2183828/70‑1 (MQ=255) tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCg > 1:2201398/1‑71 (MQ=255) cgcTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACggg < 1:565272/71‑1 (MQ=255) cgcTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACggg > 1:2009547/1‑71 (MQ=255) cTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAg < 1:1201620/60‑1 (MQ=255) aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTc < 1:2007808/71‑1 (MQ=255) tttttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGa > 1:493415/1‑71 (MQ=255) tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGt > 1:1296052/1‑66 (MQ=255) tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGt > 1:292456/1‑66 (MQ=255) cATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTa < 1:1656340/46‑1 (MQ=255) tCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGa < 1:1336201/39‑1 (MQ=255) tCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGa < 1:289800/39‑1 (MQ=255) tCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCt < 1:1472954/43‑1 (MQ=255) cAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGcgcg < 1:2157144/67‑1 (MQ=255) aGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGc < 1:524771/71‑1 (MQ=255) tAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACggg < 1:949499/49‑1 (MQ=255) tAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACggg < 1:85819/49‑1 (MQ=255) aCGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTgcgc < 1:269033/61‑1 (MQ=255) aCGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGt > 1:2091703/1‑48 (MQ=255) aCGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGt > 1:1058662/1‑48 (MQ=255) gATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGc > 1:2088579/1‑71 (MQ=255) gATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGc > 1:887077/1‑71 (MQ=255) tGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCa > 1:503226/1‑71 (MQ=255) tttCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTc > 1:1888202/1‑70 (MQ=255) ttCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACg < 1:427280/40‑1 (MQ=255) ttCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACg < 1:68354/40‑1 (MQ=255) | ATGCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTC > minE/2726106‑2726233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |