| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,732,642 | C→A | 26.6% | Q238K (CAA→AAA) | yheO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,732,642 | 0 | C | A | 26.6% | 195.6 / 34.8 | 75 | Q238K (CAA→AAA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (29/26); new base A (7/13); total (36/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGT > minE/2732573‑2732706 | cGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:885262/71‑1 (MQ=255)cGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTGCAAGAGCGGTGATTTCCAGGGGCa < 1:42554/71‑1 (MQ=255) gCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGc > 1:1922383/1‑69 (MQ=255) gCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGc > 1:1830224/1‑69 (MQ=255) cTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGa < 1:767084/71‑1 (MQ=255) tGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCTGTGATTTCCAGGGGAAAGa > 1:695469/1‑70 (MQ=255) tGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGa > 1:985663/1‑70 (MQ=255) tGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGa > 1:1966758/1‑70 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCCAGAt < 1:1243271/69‑1 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt < 1:405311/69‑1 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:407284/1‑69 (MQ=255) aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt < 1:1977724/69‑1 (MQ=255) cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2121814/71‑1 (MQ=255) cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:1422886/71‑1 (MQ=255) cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:684331/71‑1 (MQ=255) cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGt < 1:2202362/71‑1 (MQ=255) aaaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGt < 1:1895095/71‑1 (MQ=255) aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGtt < 1:1070009/71‑1 (MQ=255) aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGtt < 1:739799/71‑1 (MQ=255) aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGAGCa < 1:922092/55‑1 (MQ=255) acacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTg > 1:1051446/1‑71 (MQ=255) acacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTg > 1:1505490/1‑71 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTa > 1:1067045/1‑55 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTa > 1:1241433/1‑55 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTa > 1:2035867/1‑55 (MQ=255) cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:908589/1‑55 (MQ=255) cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:2109677/1‑55 (MQ=255) tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt > 1:1008102/1‑57 (MQ=255) tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggt > 1:1415204/1‑69 (MQ=255) ctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:1727038/1‑45 (MQ=255) ctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAc > 1:1976588/1‑71 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggtg < 1:1823605/68‑1 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:454894/64‑1 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:2157085/69‑1 (MQ=255) aCATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt > 1:1963611/1‑54 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1189174/1‑46 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1452759/1‑46 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1623304/1‑46 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1191766/1‑46 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTaa > 1:636851/1‑46 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:1470870/1‑71 (MQ=255) tCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGt < 1:55372/49‑1 (MQ=255) tCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGt < 1:1253548/49‑1 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:625557/1‑52 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:395258/1‑52 (MQ=255) gCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:1414322/71‑1 (MQ=255) cccAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt < 1:1430882/34‑1 (MQ=255) aGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:1526928/1‑70 (MQ=255) cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtgg > 1:1867743/1‑52 (MQ=255) cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGAATACgg > 1:1376663/1‑71 (MQ=255) cAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACg < 1:550887/70‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGt < 1:165313/38‑1 (MQ=255) tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:1761943/55‑1 (MQ=255) tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:2148800/55‑1 (MQ=255) tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:1148417/55‑1 (MQ=255) tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACa < 1:1721720/71‑1 (MQ=255) gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:399966/1‑45 (MQ=255) gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:1923024/1‑45 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:2047951/39‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:2045332/39‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:1613640/39‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:1422635/1‑71 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTAACCGGGCCAGCATACGGTACGCAACAgg > 1:1139765/1‑71 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATAGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:1076544/1‑71 (MQ=255) tttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:579357/43‑1 (MQ=255) tttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:1396288/43‑1 (MQ=255) tttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:893889/43‑1 (MQ=255) tttCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgtg < 1:290496/39‑1 (MQ=255) tttCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgtg < 1:189138/39‑1 (MQ=255) ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGGATACGGTACGCAACAGGc < 1:1314628/70‑1 (MQ=255) ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGc < 1:416223/70‑1 (MQ=255) tCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAcc > 1:1198030/1‑43 (MQ=255) cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:427119/1‑50 (MQ=255) cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:1210211/1‑50 (MQ=255) cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagt < 1:750703/71‑1 (MQ=255) cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagt < 1:1586766/71‑1 (MQ=255) ggCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACg > 1:2026762/1‑50 (MQ=255) | CGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGT > minE/2732573‑2732706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |