| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 335,162 | G→A | 32.0% | G130G (GGC→GGT) | ybaK ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 335,162 | 0 | G | A | 32.0% | 29.9 / 18.2 | 25 | G130G (GGC→GGT) | ybaK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/5); new base A (5/3); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTT > minE/335093‑335225 | aGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTgccgc > 1:343983/1‑71 (MQ=255) tttGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGaaa < 1:1314429/71‑1 (MQ=255) cATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATaa < 1:1518098/71‑1 (MQ=255) ggATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTg > 1:336750/1‑71 (MQ=255) cTTTGCCAGATCGCCTGCCTCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGaaa > 1:1188247/1‑56 (MQ=255) tGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCaaa < 1:2099665/69‑1 (MQ=255) tGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATAAATAGTGGCAAAtt > 1:597616/1‑71 (MQ=255) cAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATaa > 1:948138/1‑55 (MQ=255) aGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCtt > 1:1907976/1‑70 (MQ=255) aGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATAAATAGTGGCAAATTCTtg > 1:1636503/1‑71 (MQ=255) gATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATAAAt < 1:533067/55‑1 (MQ=255) tCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATAAATAg > 1:1386789/1‑55 (MQ=255) tCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGc > 1:314204/1‑71 (MQ=255) ccgccAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgtg > 1:455877/1‑63 (MQ=255) aGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGa > 1:367381/1‑70 (MQ=255) ttCGATATCCAGTCCGCGCTTGCCACCGGAAACATAAATAGTGGCaaa < 1:1787383/48‑1 (MQ=255) cGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATagt > 1:1670111/1‑68 (MQ=255) cGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAAt > 1:760864/1‑70 (MQ=255) cGATATCCAGTCCGCGCTTGCCACCGGAAACATAAATa > 1:917577/1‑38 (MQ=255) atatCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGt < 1:700539/71‑1 (MQ=255) cAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATaa > 1:474508/1‑62 (MQ=255) cAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATaa > 1:693609/1‑62 (MQ=255) ccGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCg > 1:159888/1‑45 (MQ=255) cgcgCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGtt < 1:1010434/62‑1 (MQ=255) cgcgCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGt > 1:513681/1‑71 (MQ=255) gcTTGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtttt < 1:1688048/71‑1 (MQ=255) | AGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTT > minE/335093‑335225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |