| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,802,519 | T→A | 56.7% | D196V (GAT→GTT) | qor ← | quinone oxidoreductase, NADPH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,802,519 | 0 | T | A | 56.7% | 8.1 / 38.0 | 30 | D196V (GAT→GTT) | qor | quinone oxidoreductase, NADPH‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (8/5); new base A (12/5); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01 | |||||||||||
CACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTT > minE/2802455‑2802577 | cACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCctc > 1:1298263/1‑69 (MQ=255)cACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCctc > 1:1146251/1‑69 (MQ=255) aTCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGa > 1:1042626/1‑70 (MQ=255) tACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCAc < 1:796923/64‑1 (MQ=255) tcaccacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGtt < 1:1852394/69‑1 (MQ=255) ccacGCTCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATc > 1:284699/1‑52 (MQ=255) ccacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATc > 1:1373204/1‑52 (MQ=255) cgcgCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAAc > 1:1286584/1‑70 (MQ=255) tttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAcg > 1:1443872/1‑71 (MQ=255) tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat > 1:1278118/1‑58 (MQ=255) cgcccGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGtt < 1:1848358/49‑1 (MQ=38) ggTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAAcc < 1:11163/52‑1 (MQ=255) gTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCAc < 1:1299137/37‑1 (MQ=255) gTGATCTCTTTTAACCGCTCGACCAGAACCCCTTCAc < 1:1827696/37‑1 (MQ=37) tGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTg > 1:677449/1‑52 (MQ=255) gATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcg > 1:1854718/1‑71 (MQ=255) ctctTTTAACCGCTCGACCAGATCCTCTTCACGATAGTtaataa > 1:2114780/1‑44 (MQ=255) tctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGct > 1:1106453/1‑71 (MQ=255) ctTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGctc > 1:1483354/1‑71 (MQ=255) ctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat > 1:1045529/1‑40 (MQ=255) ttttAACCGCTCGACCAGAACCTCTTCACGATAGTtaata < 1:1254863/40‑1 (MQ=255) ttttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGctct > 1:1274986/1‑71 (MQ=255) ttttAACCCCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGctct > 1:2113440/1‑71 (MQ=255) ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAAc > 1:384969/1‑40 (MQ=255) ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTgc > 1:2162805/1‑71 (MQ=255) ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTgc > 1:55439/1‑71 (MQ=255) ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTgc > 1:828125/1‑71 (MQ=255) ccGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcg < 1:1258873/59‑1 (MQ=255) cGCTCGACCAGCTCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCttt < 1:2206300/71‑1 (MQ=255) aTCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTa < 1:1425983/44‑1 (MQ=255) | CACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTT > minE/2802455‑2802577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |