| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,850,177 | G→A | 26.9% | Q286* (CAG→TAG) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,850,177 | 0 | G | A | 26.9% | 41.7 / 14.7 | 26 | Q286* (CAG→TAG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (7/12); new base A (4/3); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
ATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGC > minE/2850108‑2850236 | aTTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGt > 1:803495/1‑71 (MQ=255) gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc > 1:754848/1‑71 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCg < 1:1570215/71‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCg < 1:2191464/71‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCg < 1:210080/71‑1 (MQ=255) cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:209156/70‑1 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCTAGGCaa > 1:209689/1‑71 (MQ=255) tCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAAc > 1:1733198/1‑71 (MQ=255) gCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:1649627/70‑1 (MQ=255) tATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccatc < 1:924893/71‑1 (MQ=255) tATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccatc < 1:435684/71‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCg > 1:1769290/1‑71 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccatcc < 1:205421/68‑1 (MQ=255) cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCt > 1:211057/1‑71 (MQ=255) cTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCa < 1:339591/71‑1 (MQ=255) gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCt > 1:2205567/1‑60 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaa < 1:873986/69‑1 (MQ=255) tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAg > 1:436571/1‑71 (MQ=255) aCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgc > 1:92487/1‑70 (MQ=255) aCGTCTGGCTGGCAGCCTGACCCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc < 1:272730/71‑1 (MQ=255) gTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgc < 1:921153/71‑1 (MQ=255) gctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa < 1:308534/69‑1 (MQ=255) ggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGtt > 1:1975654/1‑71 (MQ=255) gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTc > 1:231382/1‑71 (MQ=255) aGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTcgc < 1:1821740/71‑1 (MQ=255) ccTTACGCTATTGCGAGGAAACTAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:1422075/59‑1 (MQ=38) | ATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGC > minE/2850108‑2850236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |