| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,858,845 | G→A | 41.3% | S373S (TCG→TCA) | amiB → | N‑acetylmuramoyl‑l‑alanine amidase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,858,845 | 0 | G | A | 41.3% | 27.4 / 52.4 | 46 | S373S (TCG→TCA) | amiB | N‑acetylmuramoyl‑l‑alanine amidase II |
| Reads supporting (aligned to +/- strand): ref base G (11/16); new base A (13/6); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
TGCAACGCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTG > minE/2858781‑2858908 | tGCAACGCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTGTGCGCTCACCGGa > 1:954966/1‑70 (MQ=255)tGCAACGCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGa > 1:1202243/1‑70 (MQ=255) gCAACGCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGat < 1:1561071/70‑1 (MQ=255) gCAACGCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGat > 1:1981058/1‑70 (MQ=255) gCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCa < 1:1076638/71‑1 (MQ=255) ggCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAg > 1:1864218/1‑70 (MQ=255) ggCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAg > 1:1802239/1‑70 (MQ=255) ggCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAg > 1:520384/1‑70 (MQ=255) ggCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAg > 1:561757/1‑70 (MQ=255) ggCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAg > 1:66982/1‑70 (MQ=255) ggCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCa > 1:1646402/1‑69 (MQ=255) ggCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAg > 1:744797/1‑70 (MQ=255) cGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTa > 1:1201533/1‑70 (MQ=255) aaTACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTAc > 1:1884994/1‑68 (MQ=255) aTACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGt > 1:1351605/1‑71 (MQ=255) tACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTc < 1:1347180/71‑1 (MQ=255) tACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGTc < 1:979270/71‑1 (MQ=255) cATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCg > 1:317605/1‑70 (MQ=255) aTAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGa > 1:138731/1‑70 (MQ=255) cGTCGACCAGAACTCGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCgg < 1:1998378/71‑1 (MQ=255) aCCAGAACACGCCAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTa < 1:1885388/71‑1 (MQ=255) ccAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTAt < 1:2133374/71‑1 (MQ=255) cAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGttt < 1:533690/67‑1 (MQ=255) cGCCAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGcaac > 1:1050580/1‑70 (MQ=255) cAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGcaacaa > 1:1850455/1‑69 (MQ=255) cAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGcaacaa > 1:2067186/1‑69 (MQ=255) cAGCCTTGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGcaacaa > 1:1863975/1‑69 (MQ=255) cAGCCTTGGCGTTCTGCGATCGCCGGATATCCCATCAGTACTGGTCGAAACCGGtttt < 1:1847386/58‑1 (MQ=255) gCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGc > 1:1878460/1‑71 (MQ=255) ccTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGcaac < 1:1367561/64‑1 (MQ=255) ccTTGGCGTTCTGCCCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCg < 1:263296/71‑1 (MQ=255) ttGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTa > 1:520942/1‑54 (MQ=255) ttGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCa > 1:242295/1‑57 (MQ=255) ttGGCGTTCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCg > 1:1650947/1‑69 (MQ=255) cGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAAc < 1:575642/71‑1 (MQ=255) ttCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGtttt < 1:2066847/47‑1 (MQ=255) ttCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAAc < 1:1878937/69‑1 (MQ=255) ttCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGt < 1:1207565/71‑1 (MQ=255) ttCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCgaa > 1:1802954/1‑65 (MQ=255) ttCTGCGCTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGt < 1:208781/71‑1 (MQ=255) ttCTGCGATCGCCGGATATCCCATCAGTACTGGTCGAAACCGGtttt < 1:2175467/47‑1 (MQ=255) tGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGtttt < 1:1872838/44‑1 (MQ=255) tGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGtttt < 1:1227403/44‑1 (MQ=255) cgcTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGttt < 1:8537/68‑1 (MQ=255) cgcTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTg < 1:47774/69‑1 (MQ=255) cgcTCACCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTg < 1:45217/69‑1 (MQ=255) tagCCGGTTATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGcaac < 1:2200547/46‑1 (MQ=39) | TGCAACGCATTGGCGAAATACATAAACGTCGACCAGAACACGCCAGCCTTGGCGTTCTGCGCTCGCCGGATATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTG > minE/2858781‑2858908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |