| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,864,584 | C→T | 24.1% | A295A (GCC→GCT) | hflK → | modulator for HflB protease specific for phage lambda cII repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,864,584 | 0 | C | T | 24.1% | 51.3 / 14.6 | 29 | A295A (GCC→GCT) | hflK | modulator for HflB protease specific for phage lambda cII repressor |
| Reads supporting (aligned to +/- strand): ref base C (4/18); new base T (4/3); total (8/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
CCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAG > minE/2864514‑2864648 | ccAACGAAGTTCAGCCGCGTGCGAACGGTCAGTCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGcc > 1:1576258/1‑71 (MQ=255) aaCGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCa < 1:2082847/71‑1 (MQ=255) aaCGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCa < 1:1787366/71‑1 (MQ=255) gAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGAc > 1:2118823/1‑71 (MQ=255) aGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGa > 1:388154/1‑68 (MQ=255) gTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCAt < 1:731095/71‑1 (MQ=255) tCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATcc > 1:1614368/1‑71 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATcc < 1:1003668/70‑1 (MQ=255) gcgTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCa < 1:2079136/62‑1 (MQ=255) cGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCa < 1:1855088/70‑1 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGa < 1:2171794/71‑1 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTg > 1:106255/1‑70 (MQ=255) aGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTg < 1:1781382/71‑1 (MQ=255) aGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTAAAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTg < 1:1404117/71‑1 (MQ=255) aaCGTATCCTCGAAGAGTCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:2181329/61‑1 (MQ=255) aaCGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCa < 1:1994513/37‑1 (MQ=255) aaCGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:250894/61‑1 (MQ=255) cTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATcc > 1:751190/1‑37 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAAc > 1:1527877/1‑70 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACt > 1:1679797/1‑71 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACt > 1:205711/1‑71 (MQ=255) agagGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTc < 1:815369/71‑1 (MQ=255) cgtgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCgg < 1:1869634/70‑1 (MQ=255) gtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAatat < 1:1360471/70‑1 (MQ=255) gtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAAtata < 1:1100685/71‑1 (MQ=255) tACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTa < 1:226124/52‑1 (MQ=255) aCAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTc < 1:2072152/57‑1 (MQ=255) cAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTa < 1:1333468/50‑1 (MQ=255) cAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAg < 1:703891/71‑1 (MQ=255) cAAGGCCCAGAACATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAg < 1:785830/71‑1 (MQ=255) | CCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAG > minE/2864514‑2864648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |