| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,912,429 | C→T | 23.9% | A135T (GCG→ACG) | pyrI ← | aspartate carbamoyltransferase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,912,429 | 0 | C | T | 23.9% | 91.1 / 23.8 | 46 | A135T (GCG→ACG) | pyrI | aspartate carbamoyltransferase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (13/22); new base T (6/5); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTG > minE/2912363‑2912499 | aCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:261017/1‑69 (MQ=255) cGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat > 1:738073/1‑70 (MQ=255) cAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt < 1:135103/71‑1 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg > 1:432723/1‑71 (MQ=255) ttGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc < 1:754081/68‑1 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat > 1:718728/1‑58 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat > 1:4802/1‑58 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt < 1:1744947/71‑1 (MQ=255) aGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCg > 1:1792864/1‑71 (MQ=255) aGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCCTTTTCg < 1:284599/71‑1 (MQ=255) gCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGa < 1:1260931/51‑1 (MQ=255) caccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCa > 1:606914/1‑71 (MQ=255) caccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa < 1:894435/50‑1 (MQ=255) accacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCAc > 1:8927/1‑71 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:321195/1‑70 (MQ=255) caTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCTCGATATc < 1:1607534/49‑1 (MQ=255) caTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGc > 1:1862747/1‑70 (MQ=255) ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat < 1:2037611/46‑1 (MQ=255) ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:564482/47‑1 (MQ=255) tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCa > 1:1546264/1‑47 (MQ=255) tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaaac > 1:433307/1‑70 (MQ=255) tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:563178/1‑71 (MQ=255) aTGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGc > 1:1460679/1‑71 (MQ=255) tGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGtttt > 1:1719454/1‑57 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1947752/42‑1 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1695175/42‑1 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:448748/42‑1 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg < 1:673316/71‑1 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGCCAAAGCTGGATg < 1:593037/71‑1 (MQ=255) aaCTCTTTTTCACAGTATTTGCATTTGAGAGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:2046234/71‑1 (MQ=255) aCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaa < 1:1964004/71‑1 (MQ=255) aCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:1271266/70‑1 (MQ=255) ctctTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaaa > 1:906837/1‑71 (MQ=255) tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCg > 1:1880122/1‑71 (MQ=255) tttttCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:39912/36‑1 (MQ=255) tttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc < 1:759058/68‑1 (MQ=255) tttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCg < 1:1164606/69‑1 (MQ=255) tCACAGTATTTGCATTTGAGCGCGATATCATTTGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt < 1:1122150/70‑1 (MQ=255) acaGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg < 1:88153/71‑1 (MQ=255) tATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:1616031/51‑1 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCAt < 1:714266/68‑1 (MQ=255) tGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg > 1:1616690/1‑67 (MQ=255) cATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa < 1:288496/70‑1 (MQ=255) aTTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATa < 1:322211/71‑1 (MQ=255) aTTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGATGATa < 1:1742333/71‑1 (MQ=255) ttGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:367367/1‑69 (MQ=255) cgATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTg > 1:2141700/1‑71 (MQ=255) | ACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTG > minE/2912363‑2912499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |