| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,948,123 | T→C | 27.5% | T40A (ACC→GCC) | yjjB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,948,123 | 0 | T | C | 27.5% | 60.1 / 26.9 | 40 | T40A (ACC→GCC) | yjjB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (16/13); new base C (5/6); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGC > minE/2948056‑2948192 | tttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaaa > 1:1065692/1‑71 (MQ=255) tACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAac < 1:1808196/70‑1 (MQ=255) aaaTCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCgg < 1:329183/70‑1 (MQ=255) aTCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATg > 1:529287/1‑71 (MQ=255) cATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATgcg > 1:636044/1‑71 (MQ=255) gCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCa < 1:357530/70‑1 (MQ=255) cGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAg < 1:34288/70‑1 (MQ=255) cGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAg < 1:1587784/70‑1 (MQ=255) gTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATa > 1:979247/1‑71 (MQ=255) ataAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACt < 1:708836/52‑1 (MQ=255) taAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATAc > 1:996003/1‑69 (MQ=255) taAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTc > 1:1585786/1‑54 (MQ=255) aaGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAg > 1:1238229/1‑71 (MQ=255) aGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaaa > 1:147950/1‑45 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAgcgc < 1:2068456/71‑1 (MQ=255) cGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGcgcg < 1:1144218/71‑1 (MQ=255) tGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCa < 1:155193/70‑1 (MQ=255) cccGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAtt > 1:306801/1‑70 (MQ=255) cccGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTg < 1:103756/71‑1 (MQ=255) ccGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGa < 1:1749100/71‑1 (MQ=255) ggggAACATAGGGATAACGGCCGCCACTGTAAACACTTTCGGATGGGCCAGATACCAGCGCGACCa < 1:1732895/65‑1 (MQ=255) cGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTg < 1:1325620/69‑1 (MQ=255) gggAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGaa > 1:1445574/1‑70 (MQ=255) ggAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGaa > 1:137336/1‑69 (MQ=255) gAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAt > 1:1806752/1‑64 (MQ=255) aaCATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATAcc < 1:785787/71‑1 (MQ=255) aaCATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATAc > 1:835323/1‑70 (MQ=255) tAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATg > 1:1987104/1‑71 (MQ=255) aGGGATAACGGCCGCCACGGTAAACACTTTCGGATg > 1:1666729/1‑36 (MQ=255) aGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTg > 1:1659293/1‑60 (MQ=255) aGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGAcc > 1:2034600/1‑56 (MQ=255) gggATAACGGCCGCCACGGTAAACACTTTCTGATGCGCCAGATACCAGCGCGa > 1:2107659/1‑53 (MQ=255) gggATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt < 1:681649/71‑1 (MQ=255) aCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGaaaa > 1:1263009/1‑55 (MQ=255) cgccACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa < 1:1960771/71‑1 (MQ=255) cgccACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa < 1:2074355/71‑1 (MQ=255) aCGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGaa > 1:1147334/1‑46 (MQ=255) aCGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGa < 1:1320729/71‑1 (MQ=255) gTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTg < 1:57756/41‑1 (MQ=255) gTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGc > 1:498561/1‑71 (MQ=255) | TTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGC > minE/2948056‑2948192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |