| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,986,542 | A→G | 19.9% | L19L (TTG→CTG) | arcA ← | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,986,542 | 0 | A | G | 19.9% | 113.1 / 23.7 | 54 | L19L (TTG→CTG) | arcA | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Reads supporting (aligned to +/- strand): ref base A (20/23); new base G (3/7); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTA > minE/2986473‑2986608 | tCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAAc > 1:1225905/1‑71 (MQ=255) tGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGtt < 1:1186229/71‑1 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg < 1:1862607/70‑1 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc > 1:1682736/1‑71 (MQ=255) tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCgt < 1:1810332/71‑1 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtg < 1:1560071/70‑1 (MQ=255) gcgcCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAc < 1:768294/71‑1 (MQ=255) gcgcCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAc < 1:209367/71‑1 (MQ=255) gcgcCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAc < 1:2093047/71‑1 (MQ=255) cgcCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc < 1:1286350/71‑1 (MQ=255) tCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACt > 1:1913679/1‑70 (MQ=255) tCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc > 1:425315/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGt > 1:780400/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGt > 1:546437/1‑71 (MQ=255) tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCaa > 1:1731080/1‑64 (MQ=255) tCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:99055/49‑1 (MQ=255) cGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg < 1:886853/67‑1 (MQ=255) gCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgtgt > 1:920760/1‑49 (MQ=255) gCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTc < 1:1782035/71‑1 (MQ=255) ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTc < 1:225259/69‑1 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:1459172/1‑70 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc < 1:501526/70‑1 (MQ=255) gAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa < 1:963523/71‑1 (MQ=255) aaaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCa > 1:1143601/1‑65 (MQ=255) catcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGa < 1:480807/71‑1 (MQ=255) tcatAGCCTTCCGCTTCGAAAATACTTTTCAACgtgt > 1:2028752/1‑37 (MQ=255) tcatAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:2124758/35‑1 (MQ=255) catAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg < 1:1696955/53‑1 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg < 1:1882693/52‑1 (MQ=255) tAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGaa < 1:984659/67‑1 (MQ=255) gCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCg < 1:1832618/71‑1 (MQ=255) gCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCTTCTTCAACGATAAGAATGTGCg > 1:1439767/1‑71 (MQ=255) cTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCggg < 1:1010438/71‑1 (MQ=255) ttCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtgtg < 1:2131307/66‑1 (MQ=255) tCCGCTTCGAAAATACATTTCAACGTGTTGAGTCTTACCAACTCGTCTTCAACGATa > 1:1714275/1‑57 (MQ=255) ccGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGt > 1:1385889/1‑70 (MQ=255) ccGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGt > 1:2104864/1‑70 (MQ=255) cGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCt < 1:2137459/71‑1 (MQ=255) tCGAAAATACTTTTCATCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCAt > 1:2206205/1‑71 (MQ=255) aaaaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGtt < 1:1072759/71‑1 (MQ=255) aaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg > 1:877303/1‑71 (MQ=255) aaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg > 1:752308/1‑71 (MQ=255) aTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGc < 1:965826/71‑1 (MQ=255) aTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCtgg > 1:1438436/1‑57 (MQ=255) aTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCggg > 1:711192/1‑57 (MQ=255) aTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCggg > 1:397169/1‑57 (MQ=255) aTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGttt > 1:1297955/1‑69 (MQ=255) tACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCt < 1:93667/71‑1 (MQ=255) tACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCt < 1:1668564/71‑1 (MQ=255) tACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCt < 1:1673173/71‑1 (MQ=255) aCTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:129631/1‑42 (MQ=255) ttttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt < 1:983573/63‑1 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGc > 1:2114122/1‑65 (MQ=255) tCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTa < 1:19054/70‑1 (MQ=255) | TCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTA > minE/2986473‑2986608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |