| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 498,441 | C→T | 26.6% | G24S (GGT→AGT) | galE ← | UDP‑galactose‑4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 498,441 | 0 | C | T | 26.6% | 111.4 / 43.2 | 64 | G24S (GGT→AGT) | galE | UDP‑galactose‑4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (25/22); new base T (7/10); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01 | |||||||||||
AACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATA > minE/498372‑498511 | aaCGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGGTGACATCATGACCg > 1:499824/1‑71 (MQ=255)aaCGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGAcc < 1:1448583/70‑1 (MQ=255) aCGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGt < 1:1344557/71‑1 (MQ=255) cTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtttt > 1:773786/1‑71 (MQ=255) cTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGt < 1:1491230/68‑1 (MQ=255) gATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTg > 1:1641992/1‑69 (MQ=255) gATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGc < 1:2204192/70‑1 (MQ=255) tAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGt < 1:614411/71‑1 (MQ=255) tAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:213831/71‑1 (MQ=255) tAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:309262/71‑1 (MQ=255) aaCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt > 1:1800972/1‑70 (MQ=255) cAGGAAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAAt > 1:1623798/1‑71 (MQ=255) ggCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTg > 1:525542/1‑71 (MQ=255) gTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTa < 1:609357/63‑1 (MQ=255) gTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTa < 1:2091418/63‑1 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcaca > 1:65329/1‑70 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGcaca < 1:1640/70‑1 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCatt > 1:727866/1‑57 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt > 1:2077341/1‑59 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTaa > 1:82771/1‑61 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTaa > 1:707111/1‑61 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:2060807/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:1463108/1‑71 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTaa > 1:1806226/1‑56 (MQ=255) aCTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACt < 1:523921/69‑1 (MQ=255) aCTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGGTTTGCAGTAATTGCACACAGGTATGACt < 1:981964/69‑1 (MQ=255) aCTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCATTAATTGcacac < 1:2024065/59‑1 (MQ=255) aCTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGAc > 1:2167736/1‑68 (MQ=255) ttACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCa < 1:1875238/69‑1 (MQ=255) cAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTa < 1:1913766/71‑1 (MQ=255) agGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAAcc > 1:1053035/1‑71 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGt < 1:856808/36‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGt < 1:2070805/36‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGt < 1:597033/36‑1 (MQ=255) ttATCAAGAACGATGACATCATGACTGTTTTGCAGt < 1:1950524/36‑1 (MQ=38) tATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTa > 1:1477749/1‑71 (MQ=255) tCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac < 1:166722/70‑1 (MQ=255) aaGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTaccac > 1:493073/1‑71 (MQ=255) aaGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTaccac > 1:201570/1‑71 (MQ=255) gAATGATGACATCATGACCGTTTTGCAGTAATTGcacac < 1:715805/39‑1 (MQ=255) gAATGATGACATCATGACCGTTTTGCAGTAATTGcacac < 1:284958/39‑1 (MQ=255) gAATGATGACATCATGACCGTTTTGCAGTAATTGcacac < 1:901216/39‑1 (MQ=255) aaTGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTa > 1:931721/1‑43 (MQ=255) atgatgACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGt < 1:1132651/71‑1 (MQ=255) tgatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCg > 1:1717560/1‑68 (MQ=255) tgatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCg > 1:1680178/1‑68 (MQ=255) tgatgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGt < 1:1540451/70‑1 (MQ=255) atgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc > 1:1092540/1‑71 (MQ=255) atgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc > 1:450558/1‑71 (MQ=255) atgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc > 1:2210347/1‑71 (MQ=255) atgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc < 1:1603530/71‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc > 1:1441431/1‑69 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc > 1:1632238/1‑69 (MQ=255) tcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc < 1:1667817/65‑1 (MQ=255) tcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGaa < 1:660313/70‑1 (MQ=255) catGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc < 1:1782442/64‑1 (MQ=255) atGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActc > 1:997554/1‑71 (MQ=255) atGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActc > 1:2073814/1‑71 (MQ=255) atGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCaa > 1:1273757/1‑41 (MQ=255) atGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:1175156/1‑53 (MQ=255) atGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActc > 1:1973798/1‑71 (MQ=255) tGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAActct < 1:1623641/71‑1 (MQ=255) aCCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACtctc < 1:631281/70‑1 (MQ=255) aCCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACtctc < 1:164175/70‑1 (MQ=255) aCCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCa < 1:1683650/71‑1 (MQ=255) ccGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCa > 1:1152461/1‑70 (MQ=255) ccGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCa < 1:419668/70‑1 (MQ=255) cGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATa < 1:318545/71‑1 (MQ=255) | AACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCATA > minE/498372‑498511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |