| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 507,425 | C→A | 16.3% | A71D (GCT→GAT) | ybhH → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 507,425 | 0 | C | A | 16.3% | 188.8 / 14.2 | 67 | A71D (GCT→GAT) | ybhH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (36/20); new base A (3/8); total (39/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01 | |||||||||||
ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTG > minE/507356‑507491 | aCGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:594563/71‑1 (MQ=255) gTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa > 1:1444749/1‑70 (MQ=255) ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTc > 1:1484277/1‑71 (MQ=255) ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTc > 1:1094162/1‑71 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa > 1:1643407/1‑71 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa > 1:668829/1‑71 (MQ=255) ggTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCt < 1:530867/71‑1 (MQ=255) gTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTg < 1:904493/71‑1 (MQ=255) tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt < 1:479779/71‑1 (MQ=255) tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt < 1:600602/71‑1 (MQ=255) tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt < 1:1187740/71‑1 (MQ=255) tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGt < 1:2025606/71‑1 (MQ=255) tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGt < 1:2146481/71‑1 (MQ=255) aTCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa > 1:1681516/1‑55 (MQ=255) aTCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa > 1:22913/1‑55 (MQ=255) tCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTg < 1:684670/71‑1 (MQ=255) tCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTg < 1:529354/71‑1 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGtt > 1:1015053/1‑67 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgcg > 1:12881/1‑71 (MQ=255) gACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAg > 1:403515/1‑70 (MQ=255) ccAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCt > 1:1866028/1‑58 (MQ=255) cAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:213584/43‑1 (MQ=255) aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt > 1:1419817/1‑71 (MQ=255) aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt > 1:309958/1‑71 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:420205/1‑51 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:1643796/1‑51 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:1936139/1‑51 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:771432/1‑51 (MQ=255) aaGTCGCCATTATTAGCCGTTACAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCg > 1:63483/1‑69 (MQ=255) tCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt < 1:1628770/71‑1 (MQ=255) cGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:1362622/1‑70 (MQ=255) gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAgg > 1:198633/1‑58 (MQ=255) gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:520425/1‑69 (MQ=255) gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:848173/1‑69 (MQ=255) gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa < 1:39850/71‑1 (MQ=255) attattAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAg > 1:313720/1‑69 (MQ=255) attattAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc > 1:301351/1‑70 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa < 1:2022894/71‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:477524/1‑71 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGttt > 1:1223329/1‑45 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGttt > 1:1243218/1‑45 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc < 1:1616689/70‑1 (MQ=255) aGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:1250102/1‑62 (MQ=255) gCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc < 1:1084232/71‑1 (MQ=255) gCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc < 1:1031246/71‑1 (MQ=255) gCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACgtgt < 1:469591/70‑1 (MQ=255) gCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACgtgt < 1:997419/70‑1 (MQ=255) ccGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAg < 1:362347/61‑1 (MQ=255) cGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGa < 1:1171179/71‑1 (MQ=255) gTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGAt < 1:2118205/71‑1 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:1012831/1‑55 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATa > 1:380399/1‑69 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:1425501/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:813307/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:395138/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:1700392/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc < 1:388201/70‑1 (MQ=255) ccAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc < 1:1331863/70‑1 (MQ=255) ccAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc < 1:1198880/70‑1 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc < 1:755013/59‑1 (MQ=255) aGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAg > 1:2110944/1‑54 (MQ=255) gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg > 1:1997233/1‑71 (MQ=255) gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg < 1:480219/71‑1 (MQ=255) gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg > 1:1115571/1‑71 (MQ=255) gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg > 1:1106843/1‑71 (MQ=255) tCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTa < 1:680770/71‑1 (MQ=255) cgTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTg > 1:1720346/1‑71 (MQ=255) | ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTG > minE/507356‑507491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |