| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 558,918 | T→C | 28.0% | P55P (CCT→CCC) | cmr → | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 558,918 | 0 | T | C | 28.0% | 33.1 / 16.0 | 25 | P55P (CCT→CCC) | cmr | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (7/11); new base C (4/3); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
ATATGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCT > minE/558854‑558977 | atatGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTc < 1:116687/70‑1 (MQ=255)atatGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTc < 1:134929/70‑1 (MQ=255) tatGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCg < 1:576695/70‑1 (MQ=255) tGATTCAACCCGGTATGTTGTCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGATg < 1:57165/71‑1 (MQ=255) aTTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGAc > 1:2066880/1‑71 (MQ=255) gTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTg > 1:349483/1‑71 (MQ=255) gCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGgcgggcgg > 1:1216877/1‑71 (MQ=255) aaCAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGa > 1:1941440/1‑42 (MQ=255) aaCAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTa < 1:674788/71‑1 (MQ=255) aCAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTa > 1:1545093/1‑70 (MQ=255) aaTATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACaa < 1:1706724/71‑1 (MQ=255) atCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTAc < 1:305816/66‑1 (MQ=255) aGGCGGGCATTGATTGGGTTCCCACTTCGATGACCGCGTATCTGGCGGGCTGGATGTTTTTAc < 1:381699/63‑1 (MQ=255) gCGGGCATTGATTGGGTTCCCACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACaa > 1:380302/1‑63 (MQ=255) cATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGttttt < 1:495198/54‑1 (MQ=255) ttgattgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGttttt < 1:901820/52‑1 (MQ=255) ttgattgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGtttt < 1:1361961/51‑1 (MQ=255) gattgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCt > 1:707504/1‑71 (MQ=255) gattgGGTTCCCACTTCGATGACCGCGTATCTggcg > 1:170009/1‑36 (MQ=255) gattgGGTTCCCACTTCGATGACCGCGTATCTggcg > 1:396799/1‑36 (MQ=255) gattgGGTTCCCACTTCGATGACCGCGTATCTggcg > 1:1770620/1‑36 (MQ=255) gTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGgc < 1:1442965/52‑1 (MQ=255) gTTCCCACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATg < 1:1403668/50‑1 (MQ=255) ttCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTAc < 1:2080588/45‑1 (MQ=255) ccTACTTCGATGACCGCGTATCTGGCGAGCGGGATGTTTTTACaa > 1:202320/1‑45 (MQ=255) tACTTCGATGACCGCGTATCTGGCGGGCGGGATGtttt > 1:2049659/1‑38 (MQ=255) tACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTAc < 1:934381/41‑1 (MQ=255) | ATATGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCT > minE/558854‑558977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |