| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 575,416 | T→C | 36.4% | N135S (AAT→AGT) | ybjX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 575,416 | 0 | T | C | 36.4% | 14.3 / 20.2 | 22 | N135S (AAT→AGT) | ybjX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (9/5); new base C (5/3); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
ATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGGTAACGT > minE/575347‑575478 | atGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTAtt < 1:739176/71‑1 (MQ=255)atGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTAt > 1:1226629/1‑70 (MQ=255) gTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCa < 1:969399/70‑1 (MQ=255) gTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCa > 1:949634/1‑70 (MQ=255) cTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAAtata < 1:2069894/71‑1 (MQ=255) tCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAatat > 1:1525207/1‑69 (MQ=255) aaGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTc > 1:2057321/1‑61 (MQ=255) gAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAt > 1:244431/1‑70 (MQ=255) aCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAat > 1:1476142/1‑57 (MQ=255) aCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAAttct > 1:1598193/1‑71 (MQ=255) aCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc < 1:1797999/70‑1 (MQ=255) tGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATaa < 1:369113/59‑1 (MQ=255) cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttcttc > 1:67318/1‑64 (MQ=255) tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGacata > 1:1848189/1‑70 (MQ=255) ccAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAata > 1:66827/1‑37 (MQ=255) cAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCgg < 1:1454907/70‑1 (MQ=255) ttCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTaa > 1:7030/1‑71 (MQ=255) tCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGaa > 1:1032120/1‑40 (MQ=255) ccAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCa < 1:1830535/71‑1 (MQ=255) gCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcgcg > 1:1859735/1‑55 (MQ=255) gCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcgcg > 1:1224885/1‑55 (MQ=255) cccGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaac > 1:630299/1‑71 (MQ=255) cGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaacgt < 1:1014394/71‑1 (MQ=255) cGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaacgt < 1:1870861/71‑1 (MQ=255) | ATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGGTAACGT > minE/575347‑575478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |