| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 91,462 | T→C | 23.1% | S56P (TCT→CCT) | lpxC → | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 91,462 | 0 | T | C | 23.1% | 71.1 / 21.0 | 39 | S56P (TCT→CCT) | lpxC | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase |
| Reads supporting (aligned to +/- strand): ref base T (16/14); new base C (2/7); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
ACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAG > minE/91397‑91530 | acacCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCtgtg > 1:1687204/1‑71 (MQ=255) ccGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGt < 1:1075642/71‑1 (MQ=255) ccGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt < 1:1631589/71‑1 (MQ=255) cGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGt < 1:1469375/70‑1 (MQ=255) cGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt < 1:17963/70‑1 (MQ=255) tCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATAc > 1:426880/1‑70 (MQ=255) cATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCa > 1:310789/1‑71 (MQ=255) aTCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCAt > 1:1525892/1‑71 (MQ=255) tCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATg > 1:825639/1‑71 (MQ=255) tATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGct > 1:388848/1‑71 (MQ=255) tATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGc > 1:1561389/1‑70 (MQ=255) tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGct < 1:1039879/69‑1 (MQ=255) tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGct < 1:1468591/69‑1 (MQ=255) tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGc < 1:825290/68‑1 (MQ=255) tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGc < 1:822597/68‑1 (MQ=255) gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTg > 1:944280/1‑70 (MQ=255) gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGt < 1:1365998/71‑1 (MQ=255) tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGt < 1:46957/70‑1 (MQ=255) cACCGACTTGAATCCACCGGTATATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACgt > 1:347512/1‑71 (MQ=255) cTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTgg > 1:1751814/1‑71 (MQ=255) cTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTgg > 1:688983/1‑71 (MQ=255) cTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTg < 1:1496209/70‑1 (MQ=255) ttGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGt < 1:2107249/45‑1 (MQ=255) gAATCCACCTGTAGATTTCCCGGCCGATGCCAAATCTGTGCGt < 1:65972/43‑1 (MQ=255) gAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTc < 1:2114811/70‑1 (MQ=255) aGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg > 1:1591581/1‑71 (MQ=255) aGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg > 1:1354776/1‑71 (MQ=255) gATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatga > 1:1979193/1‑68 (MQ=255) gATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg < 1:243205/70‑1 (MQ=255) gATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg < 1:555142/70‑1 (MQ=255) gATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg < 1:1486384/70‑1 (MQ=255) aTTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatg < 1:344290/66‑1 (MQ=255) tCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACg < 1:1782699/70‑1 (MQ=255) gCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTg < 1:1493753/45‑1 (MQ=255) gCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg > 1:2006789/1‑70 (MQ=255) aaaTCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGt < 1:1489812/68‑1 (MQ=255) aaaTCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTag > 1:1689578/1‑70 (MQ=255) aaTCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg > 1:579561/1‑51 (MQ=255) aaTCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTagag > 1:2045664/1‑71 (MQ=255) | ACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAG > minE/91397‑91530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |