| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 597,552 | C→T | 19.7% | intergenic (+133/‑106) | serS → / → dmsA | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine/dimethyl sulfoxide reductase, anaerobic, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 597,552 | 0 | C | T | 19.7% | 151.0 / 29.0 | 66 | intergenic (+133/‑106) | serS/dmsA | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine/dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (33/20); new base T (8/5); total (41/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTAC > minE/597488‑597622 | taatTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat > 1:382251/1‑69 (MQ=255) aatTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAattat > 1:1249065/1‑71 (MQ=255) ttACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTc < 1:702437/71‑1 (MQ=255) tcctcACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg > 1:1005625/1‑71 (MQ=255) ctcACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta > 1:1565488/1‑71 (MQ=255) cACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGtata > 1:1702382/1‑71 (MQ=255) cACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGtata > 1:1400031/1‑71 (MQ=255) cACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGtata > 1:1402483/1‑71 (MQ=255) cACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGtata > 1:1553718/1‑71 (MQ=255) cACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGtat > 1:847557/1‑70 (MQ=255) aCTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCa < 1:658257/54‑1 (MQ=255) aCTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta < 1:474940/68‑1 (MQ=255) ttACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCaaa > 1:247508/1‑54 (MQ=255) acacGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc > 1:2060016/1‑71 (MQ=255) acacGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc > 1:414889/1‑71 (MQ=255) acGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCt < 1:557249/71‑1 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCta < 1:1750073/71‑1 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGcc > 1:2169223/1‑69 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta > 1:504270/1‑71 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta < 1:926970/71‑1 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta > 1:2171745/1‑71 (MQ=255) gTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta < 1:1632700/70‑1 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:855856/1‑71 (MQ=255) aaTACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1037203/1‑70 (MQ=255) tactacTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCg < 1:403372/51‑1 (MQ=255) tactacTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCg < 1:959570/51‑1 (MQ=255) ctactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTc > 1:1655664/1‑48 (MQ=255) tactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1225869/1‑65 (MQ=255) tactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:2085039/1‑65 (MQ=255) tttCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:1094580/67‑1 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:150065/1‑71 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:287919/1‑71 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:365557/1‑71 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTCaaa > 1:1152158/1‑36 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGtata > 1:1966021/1‑50 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:634282/1‑71 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc > 1:364404/1‑71 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg < 1:422594/41‑1 (MQ=255) gAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCt < 1:1120637/66‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1031363/1‑53 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:326385/58‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:1424708/58‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:727307/58‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGa < 1:1498631/70‑1 (MQ=255) aaTCTACCTATCTCTTAGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAg > 1:1729619/1‑67 (MQ=255) cTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCta > 1:1384285/1‑46 (MQ=255) cTACCTATCTCTTTGATTTTTAAATTATTCGATGTATAAAAGCCta > 1:1509938/1‑46 (MQ=255) taacTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaat < 1:1839182/64‑1 (MQ=255) tACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGcc < 1:2134689/43‑1 (MQ=255) tACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:80426/1‑54 (MQ=255) tACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:1596873/1‑54 (MQ=255) aCCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata < 1:1448138/46‑1 (MQ=255) ccTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTa > 1:331628/1‑70 (MQ=255) ccTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTa < 1:1789532/70‑1 (MQ=255) aTCTCTTTTATTTTCAAATTATTCGATGTATACAAGCCta < 1:1564497/40‑1 (MQ=255) aTCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAAc > 1:147264/1‑50 (MQ=255) aTCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAAc > 1:1050988/1‑50 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATa > 1:2096977/1‑71 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATa > 1:863494/1‑71 (MQ=255) tttGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataa > 1:999994/1‑59 (MQ=255) tttGATTTTTAAATTATTCGATGTATACAAGCATATATAGCGAACTGCTATAGAaataa > 1:2117874/1‑59 (MQ=255) tttGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg < 1:2135562/42‑1 (MQ=255) tttGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCAAACTGCTATAGAAATAATTACACAATa < 1:610250/69‑1 (MQ=255) tGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGt > 1:1159569/1‑71 (MQ=255) ttttTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCtata < 1:446039/47‑1 (MQ=255) ttttTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCtata < 1:1712063/47‑1 (MQ=255) cAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTAc > 1:1779971/1‑71 (MQ=255) | TAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTAC > minE/597488‑597622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |