| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 623,375 | C→T | 20.0% | T19I (ACC→ATC) | msbA → | fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 623,375 | 0 | C | T | 20.0% | 65.1 / 10.9 | 30 | T19I (ACC→ATC) | msbA | fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (11/13); new base T (2/4); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATA > minE/623306‑623443 | tGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:339247/1‑70 (MQ=255) tGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCa < 1:551646/69‑1 (MQ=255) ttttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGcc > 1:89500/1‑71 (MQ=255) ttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCt < 1:1962092/70‑1 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg < 1:1459479/70‑1 (MQ=255) aTAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCg > 1:94520/1‑71 (MQ=255) aTAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGAGGCCAACCATTGCGCCTTTCAAAGCg > 1:2014065/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCa > 1:551903/1‑51 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGt > 1:1068045/1‑71 (MQ=255) cAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTg < 1:614151/70‑1 (MQ=255) aTCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaa < 1:1269579/55‑1 (MQ=255) aTCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaa < 1:281611/55‑1 (MQ=255) ctctctACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc > 1:1602604/1‑71 (MQ=255) ctctctACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTg > 1:1983111/1‑69 (MQ=255) tctACGTGGCAGACATTCCGCCGACTGTGGCCAACCa > 1:1552309/1‑37 (MQ=255) tctACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTg < 1:728598/66‑1 (MQ=255) tctACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTg < 1:1893367/66‑1 (MQ=255) tACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg > 1:1445186/1‑71 (MQ=255) tACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcggg < 1:1751334/69‑1 (MQ=255) tACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcggg < 1:802822/69‑1 (MQ=255) gCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGt < 1:2131686/71‑1 (MQ=255) gCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCa < 1:2029314/41‑1 (MQ=255) gCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGt > 1:1655211/1‑71 (MQ=255) cATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATc < 1:86898/71‑1 (MQ=255) cATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTaa > 1:39191/1‑69 (MQ=255) tCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCt > 1:58579/1‑70 (MQ=255) ccgccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc < 1:1493457/70‑1 (MQ=255) tgGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagcc < 1:1321516/69‑1 (MQ=255) cAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagccagc < 1:172619/68‑1 (MQ=255) aaCCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATa > 1:1637229/1‑71 (MQ=255) aCCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagccagc < 1:660555/66‑1 (MQ=255) | TGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATA > minE/623306‑623443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |