| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 656,044 | T→C | 26.5% | F44L (TTC→CTC) | ycbS → | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 656,044 | 0 | T | C | 26.5% | 77.9 / 33.0 | 49 | F44L (TTC→CTC) | ycbS | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base T (20/16); new base C (7/6); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACG > minE/655976‑656106 | gtggGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCtttttt < 1:1051245/70‑1 (MQ=255) tggGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCa < 1:2096009/71‑1 (MQ=255) tggGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCa < 1:2114898/71‑1 (MQ=255) ggCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaaa < 1:962655/71‑1 (MQ=255) ggCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaa < 1:528101/70‑1 (MQ=255) cTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAg < 1:2029191/70‑1 (MQ=255) gtggtgTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatga < 1:911895/71‑1 (MQ=255) gtgtTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATc > 1:555091/1‑70 (MQ=255) tttGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTc < 1:1193308/57‑1 (MQ=255) tttGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTc < 1:138402/71‑1 (MQ=255) tttGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTc < 1:741583/71‑1 (MQ=255) tgtgTCGGGAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCaa > 1:1265127/1‑71 (MQ=255) gTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtg > 1:1200930/1‑61 (MQ=255) gTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtg > 1:1408396/1‑61 (MQ=255) gTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCACAAt > 1:575675/1‑60 (MQ=255) ttCAATGCATAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAg > 1:1059754/1‑46 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaaa < 1:1149640/45‑1 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaaa < 1:195678/45‑1 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAg > 1:1858299/1‑46 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGcc < 1:1936620/66‑1 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:203272/1‑70 (MQ=255) tGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAt < 1:630401/54‑1 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatgat > 1:1710497/1‑44 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatgat > 1:1693029/1‑44 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatga > 1:232718/1‑43 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt > 1:83702/1‑64 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt > 1:831984/1‑64 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTCTCaaa > 1:1782643/1‑38 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGt > 1:1018730/1‑71 (MQ=255) caAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAgat < 1:839206/39‑1 (MQ=255) aaGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTc < 1:1484130/71‑1 (MQ=255) gCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatg > 1:1602541/1‑37 (MQ=255) cTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaa < 1:1577307/71‑1 (MQ=255) gAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaa < 1:987035/69‑1 (MQ=255) aaaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaa < 1:533672/69‑1 (MQ=255) aaaCCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGaaaa < 1:1099017/70‑1 (MQ=255) aaaCCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTACGaaaa < 1:284618/70‑1 (MQ=255) aaCCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAg > 1:1052816/1‑71 (MQ=255) cTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGAc < 1:199145/71‑1 (MQ=255) ggTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa > 1:1952688/1‑70 (MQ=255) ttCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtgg > 1:75081/1‑37 (MQ=255) ttCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtgg > 1:1234603/1‑37 (MQ=255) gATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:272917/71‑1 (MQ=255) ccTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAAc > 1:1162064/1‑71 (MQ=255) ccTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAAc > 1:1474754/1‑71 (MQ=255) cTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaaaa > 1:983626/1‑66 (MQ=255) cTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACg > 1:938523/1‑71 (MQ=255) cTGCTTTTCTCAAAGATGATCACTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACg > 1:595448/1‑71 (MQ=255) gCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATaa > 1:1020671/1‑67 (MQ=255) cTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACg > 1:2086327/1‑68 (MQ=255) | GTGGGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAACG > minE/655976‑656106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |