| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 668,428 | C→A | 24.9% | T589K (ACG→AAG) | uup → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 668,428 | 0 | C | A | 24.9% | 82.9 / 10.5 | 32 | T589K (ACG→AAG) | uup | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (18/6); new base A (4/4); total (22/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATATGGCT > minE/668361‑668498 | cAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGc < 1:1388872/70‑1 (MQ=255) gAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGc > 1:118231/1‑71 (MQ=255) agcTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGcttcc > 1:409157/1‑71 (MQ=255) cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc < 1:1685028/71‑1 (MQ=255) cAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTtcag < 1:1427468/71‑1 (MQ=255) aaTTGCTCGAAGATCTGGAGGCTAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTtcagt < 1:1864924/71‑1 (MQ=255) aaTTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTtcagt < 1:865083/71‑1 (MQ=255) aaTTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTtcagt < 1:1033926/71‑1 (MQ=255) aaTTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTtcag > 1:944954/1‑70 (MQ=255) aTTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTtcagt > 1:378317/1‑70 (MQ=255) aTTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTtcagt > 1:1754535/1‑70 (MQ=255) gCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTtcagtcag > 1:1925383/1‑70 (MQ=255) gCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTtcagtc > 1:1396776/1‑68 (MQ=255) gCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCATCTtcagtc > 1:135874/1‑68 (MQ=255) aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt > 1:1013690/1‑70 (MQ=255) gATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAg > 1:1019039/1‑37 (MQ=255) aTCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTg < 1:1214783/38‑1 (MQ=255) cTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGc > 1:1004637/1‑38 (MQ=255) cTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGc > 1:663244/1‑38 (MQ=255) aaGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaaa > 1:1841556/1‑70 (MQ=255) aaGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaaa > 1:1136541/1‑70 (MQ=255) gCTGGAAGCCCTACAGACGCAAGTGGCGTATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAg > 1:790415/1‑71 (MQ=255) aGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttg > 1:1117503/1‑71 (MQ=255) aGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttg > 1:913697/1‑71 (MQ=255) aGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgctag > 1:513007/1‑69 (MQ=255) cTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaaa < 1:1199347/58‑1 (MQ=255) tACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaaa < 1:1241580/57‑1 (MQ=255) tACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttgct < 1:2120293/68‑1 (MQ=255) aCAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttgc > 1:1701681/1‑66 (MQ=255) aCAGAAGCAAGTGGCGGATGCTTCCTTCTtcagtc > 1:1986522/1‑35 (MQ=255) cAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGatat > 1:923069/1‑71 (MQ=255) cAGACGCAAGTGGCCGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGatat > 1:1296871/1‑71 (MQ=255) cGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATATGgct > 1:104825/1‑71 (MQ=255) cGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATATGgct > 1:55944/1‑71 (MQ=255) | CAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATATGGCT > minE/668361‑668498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |