| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 696,779 | C→T | 26.3% | P262L (CCC→CTC) | appB → | cytochrome bd‑II oxidase, subunit II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 696,779 | 0 | C | T | 26.3% | 59.4 / 22.4 | 38 | P262L (CCC→CTC) | appB | cytochrome bd‑II oxidase, subunit II |
| Reads supporting (aligned to +/- strand): ref base C (17/11); new base T (2/8); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01 | |||||||||||
AATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTG > minE/696715‑696847 | aaTCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGtttt < 1:1755544/71‑1 (MQ=255) ccGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTtgtg > 1:1415494/1‑71 (MQ=255) ttAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAt < 1:560085/71‑1 (MQ=255) ttAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAt < 1:191080/71‑1 (MQ=255) aaTGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCt > 1:111229/1‑71 (MQ=255) tGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGtt < 1:983619/59‑1 (MQ=255) aCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgc > 1:1116840/1‑71 (MQ=255) tggtggCAGTGCTACCTTGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgct < 1:1968826/70‑1 (MQ=255) gtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCgctgct < 1:1882876/71‑1 (MQ=255) tGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTgg < 1:154075/66‑1 (MQ=255) tGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTgg < 1:410895/66‑1 (MQ=255) tGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCt > 1:566546/1‑54 (MQ=255) tGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttc < 1:909597/71‑1 (MQ=255) gCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttct > 1:330649/1‑71 (MQ=255) cTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGAttct > 1:149723/1‑70 (MQ=255) cTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGt < 1:1237206/41‑1 (MQ=255) cTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTtgtg > 1:236109/1‑47 (MQ=255) cTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTtgtg > 1:177544/1‑47 (MQ=255) ggTGGCTGGATGAATAATTTTGTCGAGTCGCCCGttt < 1:80364/37‑1 (MQ=255) gTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCAGCTGCTGGGATTCTTCTGCCCa > 1:418860/1‑71 (MQ=255) tGCCTGGATGAATAATTTTGTCGAGTCGCTCGtttt < 1:358514/36‑1 (MQ=255) cTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGcc > 1:24533/1‑65 (MQ=255) tGGATGAATAATTTTGTCGAGGCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc < 1:499943/60‑1 (MQ=255) gATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgct > 1:610023/1‑45 (MQ=255) gATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGa > 1:847069/1‑52 (MQ=255) gATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGa > 1:1359513/1‑52 (MQ=255) gATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTg > 1:1681984/1‑60 (MQ=255) aTGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGa > 1:76834/1‑70 (MQ=255) aataatTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:1985798/71‑1 (MQ=255) aataatTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:1553532/71‑1 (MQ=255) aataatTTTGTCGAGTCGCCCGTTTTGTGGATCtt > 1:1172008/1‑35 (MQ=255) aataatTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTggg > 1:1502084/1‑47 (MQ=255) ataatTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:474674/70‑1 (MQ=255) gAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTa < 1:2022636/71‑1 (MQ=255) aGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTAt < 1:205264/71‑1 (MQ=255) tCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCg > 1:1390111/1‑71 (MQ=255) gCCCGTGTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTg > 1:1303052/1‑71 (MQ=255) gCCAGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTg < 1:189379/71‑1 (MQ=255) | AATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTG > minE/696715‑696847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |