| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 813,910 | A→G | 26.0% | C60C (TGT→TGC) | prsA ← | phosphoribosylpyrophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 813,910 | 0 | A | G | 26.0% | 82.6 / 31.7 | 50 | C60C (TGT→TGC) | prsA | phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (15/22); new base G (9/4); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGC > minE/813845‑813977 | gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGt > 1:591546/1‑71 (MQ=255)gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGt > 1:1168802/1‑71 (MQ=255) cGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1026309/69‑1 (MQ=255) cGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAg < 1:1934981/69‑1 (MQ=255) cACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTgg < 1:1342203/71‑1 (MQ=255) gCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGatgatg < 1:1822108/71‑1 (MQ=255) aGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaa < 1:364358/71‑1 (MQ=255) cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAata < 1:1920082/71‑1 (MQ=255) aTCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAatat > 1:320377/1‑71 (MQ=255) aTCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAata > 1:1768174/1‑70 (MQ=255) tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAat < 1:1219995/68‑1 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGaaaa > 1:386017/1‑65 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGaaaa > 1:1956265/1‑65 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGaaaa > 1:863940/1‑65 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACaa > 1:1017742/1‑46 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACaa > 1:1857123/1‑46 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATcacc < 1:1594597/71‑1 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATcac > 1:1172662/1‑70 (MQ=255) ccATAACGACTAGTTCCATCAGGTTGTCGTTAGTAGGGGCACAAg > 1:19220/1‑45 (MQ=255) ccATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAat < 1:378597/64‑1 (MQ=255) ccATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAat < 1:1903803/64‑1 (MQ=255) aTAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAata > 1:925714/1‑63 (MQ=255) aTAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCaccacc > 1:1754859/1‑71 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaaaa < 1:913475/60‑1 (MQ=255) tAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaaaa < 1:812520/60‑1 (MQ=255) aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGAc > 1:781935/1‑45 (MQ=255) aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACcgc > 1:1430952/1‑70 (MQ=255) gACTAATTCCATCAGGTTGTCGTTAGTAGGGGcaca < 1:81161/36‑1 (MQ=255) gACTAATTCCATCAGGTTGTCGTTAGTAGGGGcaca < 1:47631/36‑1 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGAtgatga > 1:1035020/1‑52 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTa > 1:280883/1‑70 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTAc < 1:1661340/71‑1 (MQ=255) cTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTAc < 1:283703/70‑1 (MQ=255) tAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaaa > 1:2105689/1‑52 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGtc < 1:1404165/35‑2 (MQ=38) aTTCCATCAGGTTGTCGTTAGTAGGGGCGCAAGTg < 1:2157173/35‑1 (MQ=255) aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAatat > 1:1688681/1‑54 (MQ=255) cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAatat < 1:2002589/50‑1 (MQ=255) tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaa < 1:1259996/43‑1 (MQ=255) tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTa < 1:595607/71‑1 (MQ=255) tCAGGGTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaa < 1:2184557/43‑1 (MQ=255) aGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAt > 1:932185/1‑71 (MQ=255) ttGTCGTTAGTAGGGGCACAAGTGTACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg < 1:751137/71‑1 (MQ=255) gTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaaaa > 1:534470/1‑38 (MQ=255) gTCGTTAGTAGGGGCACAAGTGGACTGGATGATGaaa > 1:1223523/1‑37 (MQ=255) tCGTTAGTAGGGGCACAAGTGGACTGGATGATGaaa < 1:1930550/36‑1 (MQ=255) gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGc > 1:146797/1‑71 (MQ=255) agtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTg < 1:350989/70‑1 (MQ=255) agtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTg < 1:1568319/70‑1 (MQ=255) tagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGt < 1:1975443/62‑1 (MQ=255) ggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGc < 1:92431/71‑1 (MQ=255) gCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCa > 1:651889/1‑48 (MQ=255) | GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGC > minE/813845‑813977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |