| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 138,172 | A→G | 13.5% | N665N (AAT→AAC) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 138,172 | 0 | A | G | 13.5% | 84.7 / 8.1 | 37 | N665N (AAT→AAC) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (15/17); new base G (3/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
AAGTCAGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAA > minE/138102‑138226 | aaGTCAGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTa < 1:344376/71‑1 (MQ=255) cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTg > 1:1122620/1‑71 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:805729/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:1404032/70‑1 (MQ=255) cACCGCTATGCAATAC‑AAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGaa < 1:2152791/70‑1 (MQ=255) ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAAt < 1:109707/71‑1 (MQ=255) cGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATGGCTGTTTGCAGAAAtt > 1:1159777/1‑71 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTg > 1:1320777/1‑71 (MQ=255) tACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTa < 1:128942/49‑1 (MQ=255) aCAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCa > 1:535032/1‑70 (MQ=255) aCAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTAtt > 1:21342/1‑50 (MQ=255) aCAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAg > 1:860998/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGaa > 1:503905/1‑62 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTAtt > 1:214569/1‑49 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTAtt > 1:1637024/1‑49 (MQ=255) aaaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGaa > 1:771119/1‑61 (MQ=255) aaaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCg < 1:715777/71‑1 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCg < 1:851341/70‑1 (MQ=255) aaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGc < 1:1984532/64‑1 (MQ=255) ccaccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:589097/1‑58 (MQ=255) caccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTccc < 1:1313231/71‑1 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt > 1:1629531/1‑63 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt > 1:1614653/1‑63 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACt > 1:1259263/1‑70 (MQ=255) tGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt < 1:310656/62‑1 (MQ=255) tGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt < 1:489221/62‑1 (MQ=255) agagaAACTTGACGGCTGTTATCGCTATTTGCAGTAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa < 1:1588688/71‑1 (MQ=255) agagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCAt < 1:1453082/70‑1 (MQ=255) agagaAACTTGACGGATGTTATCGCTATTTGCAGAAATTGAACCTGCCAGGGTTCCCCATGGTGACTCa > 1:1209693/1‑69 (MQ=255) agagaAACTT‑ACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATgg > 1:2130939/1‑61 (MQ=255) gaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:1559575/71‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:708396/71‑1 (MQ=255) aaaCTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGt < 1:16656/59‑1 (MQ=255) ttGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:649171/1‑45 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:1858927/69‑1 (MQ=255) aCGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCAAGCGTTCCCCATGGTGACTCATAGCTGGCATaa < 1:911941/70‑1 (MQ=255) tGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt > 1:325755/1‑63 (MQ=255) ttATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTccc < 1:653766/39‑1 (MQ=255) ttATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCa < 1:344289/51‑1 (MQ=255) aTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACt < 1:587155/41‑1 (MQ=255) aTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACt < 1:890348/41‑1 (MQ=255) | AAGTCAGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAA > minE/138102‑138226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |