Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	909,573	C→T	39.5%	L584L (CTG→TTG)	ycjT →	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	909,573	0	C	T	39.5%	27.6 / 40.1	38	L584L (CTG→TTG)	ycjT	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base C (10/13);  new base T (10/5);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCAC  >  minE/909506‑909641                                                                    |                                                                     ttCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAtgttgc                                                                   <  1:938528/71‑1 (MQ=255) ttCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAtgctgc                                                                   >  1:64899/1‑71 (MQ=255)       cgcAGAAGTGAACGAGATGTAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGc                                                             <  1:1032888/71‑1 (MQ=255)            aaGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTtc                                                        <  1:646016/71‑1 (MQ=255)            aaGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTtc                                                        <  1:1170381/71‑1 (MQ=255)              gTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAGTtctc                                                      <  1:825131/71‑1 (MQ=255)              gTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTtctc                                                      <  1:765760/71‑1 (MQ=255)               tGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAGTTCTCa                                                     <  1:1212541/71‑1 (MQ=255)                 aaCGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCa                                                            >  1:96294/1‑62 (MQ=255)                 aaCGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAGTTCTCAGc                                                   >  1:12810/1‑71 (MQ=255)                 aaCGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAGTTCTCAGc                                                   >  1:1874707/1‑71 (MQ=255)                 aaCGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAg                                                    <  1:235809/70‑1 (MQ=255)                 aaCGAGATGCAGATCCTCAAACAAGCTGATCTGGTGATGCTCAATTACATGTTGCCGGAgaa                                                            >  1:1211481/1‑60 (MQ=255)                             aTCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCt                                       >  1:90819/1‑71 (MQ=255)                               ccTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGc                                                   >  1:1435236/1‑57 (MQ=255)                               ccTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATc                                             >  1:1221619/1‑63 (MQ=255)                               ccTCAAACAAGCTGATGTCGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATc                                             >  1:1498094/1‑63 (MQ=255)                               ccTCAAACAAGCTCATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGc                                                   >  1:700849/1‑57 (MQ=255)                                 tCAAACAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAg                                                           >  1:1254338/1‑47 (MQ=255)                                 tCAAACAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAg                                                           >  1:1167447/1‑47 (MQ=255)                                   aaaCAAGCTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGcc                                   <  1:1723995/69‑1 (MQ=255)                                   aaaCAAGCTGATGTGGTGATGCTCAATTACATGCTGCCTGAGCAGTTCTCAGCGGCATCGTGTCTTGCCa                                  <  1:2209482/70‑1 (MQ=255)                                     aCAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATc                               >  1:1235698/1‑71 (MQ=255)                                     aCAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATc                               >  1:1466945/1‑71 (MQ=255)                                          cTGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCaa                          <  1:630891/71‑1 (MQ=255)                                           tGATGTGGTGATGCTCAATTACATGTTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGc                                    >  1:68716/1‑60 (MQ=255)                                             aTGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAttt                       >  1:512850/1‑71 (MQ=255)                                              tgtgGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGc                                                   <  1:320595/42‑1 (MQ=255)                                                tggtgATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCaa                          <  1:1338008/65‑1 (MQ=255)                                                tggtgATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTAt                    >  1:595522/1‑71 (MQ=255)                                                tggtcATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCaa                          <  1:372062/65‑1 (MQ=255)                                                 ggtgATGCTCAATTACATGTTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAttt                       <  1:460668/67‑1 (MQ=255)                                                  gtgATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGTCATCGTGTCTTGCCAATCTGCAATTTTATg                   <  1:1128004/70‑1 (MQ=255)                                                     aTGCTCAATTACATGTTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGaa                 >  1:1567574/1‑69 (MQ=255)                                                     aTGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAAcc               <  1:586147/71‑1 (MQ=255)                                                       gCTCAATTACATGTTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACcgc             >  1:834533/1‑71 (MQ=255)                                                            aTTACATGTTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGACCCgcgcgc         >  1:1378843/1‑68 (MQ=255)                                                               aCATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTAtt     >  1:69502/1‑71 (MQ=255)                                                                cATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTAtt     <  1:1417087/70‑1 (MQ=255)                                                                cATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTAtt     <  1:503623/70‑1 (MQ=255)                                                                  tgctgcCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCAc  <  1:354475/71‑1 (MQ=255)                                                                   gctgcCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAttt                       <  1:1323185/49‑1 (MQ=255)                                                                   gctgcCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAttt                       <  1:1727693/49‑1 (MQ=255)                                                                    |                                                                     TTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCAC  >  minE/909506‑909641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑