| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,000,186 | G→A | 43.6% | G179D (GGT→GAT) | ydgD → | predicted peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,000,186 | 0 | G | A | 43.6% | 16.1 / 47.0 | 39 | G179D (GGT→GAT) | ydgD | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base G (11/11); new base A (8/9); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTG > minE/1000124‑1000254 | tACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAg > 1:1270537/1‑71 (MQ=255) aCGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGt > 1:243246/1‑71 (MQ=255) aCGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGt > 1:1186220/1‑71 (MQ=255) cGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGt > 1:1046773/1‑70 (MQ=255) cGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGt < 1:967119/70‑1 (MQ=255) cGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGt < 1:12396/70‑1 (MQ=255) cgttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGt > 1:447974/1‑62 (MQ=255) gccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTcagg > 1:1919279/1‑71 (MQ=255) gccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTcagg > 1:150109/1‑71 (MQ=255) cgttATTTGAGGGAGATAAAGCCGCACTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTcagg < 1:696163/69‑1 (MQ=255) aTTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTaaa > 1:710732/1‑55 (MQ=255) tttGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCt < 1:1692717/70‑1 (MQ=255) gggAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTg < 1:2197076/71‑1 (MQ=255) aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg < 1:1142170/66‑1 (MQ=255) aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg < 1:873216/66‑1 (MQ=255) aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg < 1:1139666/66‑1 (MQ=255) aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGa < 1:76860/70‑1 (MQ=255) tAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTa < 1:625698/41‑1 (MQ=255) aaGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGt > 1:443191/1‑38 (MQ=255) tACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGt > 1:1408210/1‑71 (MQ=255) aCCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGatcatc > 1:937225/1‑61 (MQ=255) cgcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACa < 1:1308357/71‑1 (MQ=255) ttAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtctc < 1:1258127/54‑1 (MQ=255) ttAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtctc < 1:693027/54‑1 (MQ=255) ttAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtctc < 1:513329/54‑1 (MQ=255) ttAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtctc < 1:350799/54‑1 (MQ=255) ttAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGt < 1:841917/61‑1 (MQ=255) tAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcat > 1:2048686/1‑71 (MQ=255) tAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcat > 1:1841889/1‑71 (MQ=255) aaaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGa > 1:1129324/1‑45 (MQ=255) aaaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTg > 1:1130554/1‑61 (MQ=255) aaaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcat > 1:1063910/1‑70 (MQ=255) aaaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtc > 1:1670967/1‑68 (MQ=255) aaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcatc < 1:1305553/70‑1 (MQ=255) aaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcat > 1:1823916/1‑69 (MQ=255) aaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaa < 1:614382/71‑1 (MQ=255) aaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTAcc < 1:320041/36‑1 (MQ=255) aaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaa < 1:1981377/71‑1 (MQ=255) aGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTAc > 1:1055433/1‑61 (MQ=255) gTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACg < 1:834900/47‑1 (MQ=255) gTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACg < 1:26686/47‑1 (MQ=255) gTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACg < 1:174296/47‑1 (MQ=255) gTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtgtg > 1:1783845/1‑69 (MQ=255) | TACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTG > minE/1000124‑1000254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |