Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,011,234 A→G 22.7% H221R (CAC→CGC)  rstB → sensory histidine kinase in two‑component regulatory system with RstA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,011,2340AG22.7% 80.7 / 24.1 44H221R (CAC→CGC) rstBsensory histidine kinase in two‑component regulatory system with RstA
Reads supporting (aligned to +/- strand):  ref base A (15/19);  new base G (4/6);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

TTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAGCGCCG  >  minE/1011164‑1011299
                                                                      |                                                                 
tttAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCa                                                                   >  1:708556/1‑71 (MQ=255)
  tAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCAc                                                                  >  1:2005648/1‑70 (MQ=255)
   aaCCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACg                                                                 <  1:1802833/70‑1 (MQ=255)
    aCCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGa                                                                <  1:1082935/70‑1 (MQ=255)
    aCCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGa                                                                <  1:1271160/70‑1 (MQ=255)
      cAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACg                                                                 <  1:1961428/67‑1 (MQ=255)
         aTGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGaa                                                               >  1:725849/1‑66 (MQ=255)
          tGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCg                                                          >  1:876498/1‑70 (MQ=255)
                 cAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGt                                                  <  1:1457062/71‑1 (MQ=255)
                  aaTATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGtt                                                 <  1:1303553/71‑1 (MQ=255)
                    tatCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAg                                               >  1:1937978/1‑71 (MQ=255)
                     atCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAacac                                                     >  1:644303/1‑64 (MQ=255)
                     atCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGt                                              >  1:1813327/1‑71 (MQ=255)
                      tCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTg                                             >  1:987227/1‑71 (MQ=255)
                        aaCGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTgc                                            >  1:840480/1‑70 (MQ=255)
                         aCGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGaa                                                               >  1:1211401/1‑50 (MQ=255)
                          cGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGcc                                         <  1:1809332/71‑1 (MQ=255)
                          cGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGcc                                         >  1:1233321/1‑71 (MQ=255)
                            ccTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGCCTg                                       >  1:800856/1‑71 (MQ=255)
                            ccTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGaa                                                        >  1:820489/1‑54 (MQ=255)
                                 aTTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGCCTGCGTTa                                  <  1:629910/71‑1 (MQ=255)
                                  ttGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAAc                                                              <  1:417709/42‑1 (MQ=255)
                                    gCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGaa                                                        >  1:543158/1‑46 (MQ=255)
                                     ccAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGa                              <  1:466590/71‑1 (MQ=255)
                                          aaaaaaCAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGga                         <  1:1097928/71‑1 (MQ=255)
                                          aaaaaaCAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCAACTg                           >  1:691295/1‑69 (MQ=255)
                                            aaaaCAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTg                           >  1:2087960/1‑67 (MQ=255)
                                                  gCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCg                 <  1:334270/71‑1 (MQ=255)
                                                   cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATc                                <  1:2023752/55‑1 (MQ=255)
                                                   cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa                <  1:1164204/71‑1 (MQ=255)
                                                   cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa                <  1:115293/71‑1 (MQ=255)
                                                   cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa                <  1:173471/71‑1 (MQ=255)
                                                   cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa                <  1:212699/71‑1 (MQ=255)
                                                    ttATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGCCTGCGTTa                                  <  1:632549/52‑1 (MQ=255)
                                                     tATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGAt               >  1:1341751/1‑70 (MQ=255)
                                                      aTTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATa              <  1:977546/70‑1 (MQ=255)
                                                       ttGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAAc            <  1:773458/71‑1 (MQ=255)
                                                          aCGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCt          <  1:1566613/70‑1 (MQ=255)
                                                              tATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGtt                                   <  1:304736/41‑1 (MQ=255)
                                                              tATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcg     <  1:1107504/71‑1 (MQ=255)
                                                               aTCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcgc    >  1:341780/1‑71 (MQ=255)
                                                               aTCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcgc    >  1:339178/1‑71 (MQ=255)
                                                               aTCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcgc    >  1:1292024/1‑71 (MQ=255)
                                                                tCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCg                               <  1:898762/43‑1 (MQ=255)
                                                                  gCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAGcgccg  <  1:1027985/70‑1 (MQ=255)
                                                                      |                                                                 
TTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAGCGCCG  >  minE/1011164‑1011299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: