| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,011,234 | A→G | 22.7% | H221R (CAC→CGC) | rstB → | sensory histidine kinase in two‑component regulatory system with RstA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,011,234 | 0 | A | G | 22.7% | 80.7 / 24.1 | 44 | H221R (CAC→CGC) | rstB | sensory histidine kinase in two‑component regulatory system with RstA |
| Reads supporting (aligned to +/- strand): ref base A (15/19); new base G (4/6); total (19/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
TTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAGCGCCG > minE/1011164‑1011299 | tttAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCa > 1:708556/1‑71 (MQ=255) tAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCAc > 1:2005648/1‑70 (MQ=255) aaCCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACg < 1:1802833/70‑1 (MQ=255) aCCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGa < 1:1082935/70‑1 (MQ=255) aCCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGa < 1:1271160/70‑1 (MQ=255) cAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACg < 1:1961428/67‑1 (MQ=255) aTGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGaa > 1:725849/1‑66 (MQ=255) tGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCg > 1:876498/1‑70 (MQ=255) cAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGt < 1:1457062/71‑1 (MQ=255) aaTATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGtt < 1:1303553/71‑1 (MQ=255) tatCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAg > 1:1937978/1‑71 (MQ=255) atCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAacac > 1:644303/1‑64 (MQ=255) atCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGt > 1:1813327/1‑71 (MQ=255) tCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTg > 1:987227/1‑71 (MQ=255) aaCGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTgc > 1:840480/1‑70 (MQ=255) aCGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGaa > 1:1211401/1‑50 (MQ=255) cGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGcc < 1:1809332/71‑1 (MQ=255) cGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGcc > 1:1233321/1‑71 (MQ=255) ccTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGCCTg > 1:800856/1‑71 (MQ=255) ccTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGaa > 1:820489/1‑54 (MQ=255) aTTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGCCTGCGTTa < 1:629910/71‑1 (MQ=255) ttGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCGCGAAc < 1:417709/42‑1 (MQ=255) gCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGaa > 1:543158/1‑46 (MQ=255) ccAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGa < 1:466590/71‑1 (MQ=255) aaaaaaCAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGga < 1:1097928/71‑1 (MQ=255) aaaaaaCAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCAACTg > 1:691295/1‑69 (MQ=255) aaaaCAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTg > 1:2087960/1‑67 (MQ=255) gCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCg < 1:334270/71‑1 (MQ=255) cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATc < 1:2023752/55‑1 (MQ=255) cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa < 1:1164204/71‑1 (MQ=255) cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa < 1:115293/71‑1 (MQ=255) cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa < 1:173471/71‑1 (MQ=255) cTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGa < 1:212699/71‑1 (MQ=255) ttATTGACGGTATCGCTCGCGAACTGCGAACACCGTTAGTGCGCCTGCGTTa < 1:632549/52‑1 (MQ=255) tATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGAt > 1:1341751/1‑70 (MQ=255) aTTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATa < 1:977546/70‑1 (MQ=255) ttGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAAc < 1:773458/71‑1 (MQ=255) aCGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCt < 1:1566613/70‑1 (MQ=255) tATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGtt < 1:304736/41‑1 (MQ=255) tATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcg < 1:1107504/71‑1 (MQ=255) aTCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcgc > 1:341780/1‑71 (MQ=255) aTCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcgc > 1:339178/1‑71 (MQ=255) aTCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAgcgc > 1:1292024/1‑71 (MQ=255) tCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCg < 1:898762/43‑1 (MQ=255) gCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAGcgccg < 1:1027985/70‑1 (MQ=255) | TTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAACAGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTGAGCGCCG > minE/1011164‑1011299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |