| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,026,348 | T→C | 33.3% | E175G (GAG→GGG) | malI ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,026,348 | 0 | T | C | 33.3% | 37.0 / 31.4 | 36 | E175G (GAG→GGG) | malI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (15/9); new base C (7/5); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCA > minE/1026283‑1026416 | gggTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTc < 1:1570366/71‑1 (MQ=255)gggTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTc < 1:639358/71‑1 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac > 1:241043/1‑70 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTcaac < 1:946309/70‑1 (MQ=255) cTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtg < 1:131653/65‑1 (MQ=255) cTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGc > 1:740770/1‑71 (MQ=255) cTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTcaacaa > 1:240758/1‑62 (MQ=255) cTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTcaacaa > 1:452392/1‑62 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt > 1:826139/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt > 1:1134880/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt > 1:2022064/1‑71 (MQ=255) tGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac > 1:1773088/1‑57 (MQ=255) tGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGcc > 1:1669095/1‑69 (MQ=255) ccTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACt < 1:855810/58‑1 (MQ=255) ccTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACt < 1:340240/58‑1 (MQ=255) tCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACtgt > 1:983709/1‑58 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgttg < 1:1525501/71‑1 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgttg < 1:299230/71‑1 (MQ=255) agGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCggg > 1:1673592/1‑71 (MQ=255) agGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCggg > 1:1767666/1‑71 (MQ=255) ccGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg > 1:1897386/1‑71 (MQ=255) gCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAt < 1:1559527/46‑1 (MQ=255) ttGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTcc > 1:2102871/1‑48 (MQ=255) gCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:1736918/1‑69 (MQ=255) gCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:1355379/1‑69 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGaa > 1:209843/1‑53 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg < 1:491226/56‑1 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg < 1:1233461/56‑1 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:28756/1‑68 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:1748214/1‑69 (MQ=255) cGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:1397280/71‑1 (MQ=255) aaTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:1208293/71‑1 (MQ=255) tGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATaa > 1:1868520/1‑71 (MQ=255) aTGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTgg < 1:474447/71‑1 (MQ=255) tGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATaa > 1:130761/1‑66 (MQ=255) gCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCa > 1:1167003/1‑71 (MQ=255) | GGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACTGGCA > minE/1026283‑1026416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |