| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,027,446 | A→G | 30.8% | N134S (AAC→AGC) | malX → | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,027,446 | 0 | A | G | 30.8% | 30.2 / 18.6 | 26 | N134S (AAC→AGC) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base A (9/9); new base G (4/4); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATC > minE/1027376‑1027489 | gcgGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATaa > 1:1738975/1‑71 (MQ=255) aCTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCag < 1:209211/71‑1 (MQ=255) tCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCagag > 1:1052178/1‑70 (MQ=255) cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa > 1:287880/1‑71 (MQ=255) cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCagag < 1:2061924/69‑1 (MQ=255) gACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCtt < 1:1731545/70‑1 (MQ=255) aTAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc > 1:602019/1‑71 (MQ=255) aTAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc < 1:1760587/71‑1 (MQ=255) aTAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc < 1:737154/71‑1 (MQ=255) tAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc < 1:718484/70‑1 (MQ=255) aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCa < 1:1506200/70‑1 (MQ=255) aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCa < 1:746430/70‑1 (MQ=255) gCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcg < 1:1962751/71‑1 (MQ=255) ttCTGCCAACCACGGATTCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCa < 1:1882941/64‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:1841893/1‑71 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:1485260/1‑71 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcg > 1:473532/1‑70 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcg < 1:1272354/66‑1 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATa > 1:1052549/1‑65 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGa > 1:131600/1‑71 (MQ=255) aCGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgat > 1:1739958/1‑59 (MQ=255) cGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATc < 1:805618/71‑1 (MQ=255) ccGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCt > 1:1890070/1‑37 (MQ=255) ttCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATc < 1:1418467/38‑1 (MQ=255) gAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcta > 1:194858/1‑43 (MQ=255) gAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:637755/1‑45 (MQ=255) aaTAACATCCAGAGCATTCTTTGGATCCAGTCGATCGATAc > 1:344359/1‑41 (MQ=255) aaCATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:797074/1‑35 (MQ=255) | GCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATC > minE/1027376‑1027489 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |