| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,073,671 | C→T | 41.9% | G50S (GGC→AGC) | ydhQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,073,671 | 0 | C | T | 41.9% | 14.9 / 34.8 | 31 | G50S (GGC→AGC) | ydhQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (5/13); new base T (2/11); total (7/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
CGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTA > minE/1073603‑1073735 | cGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAAccagccag > 1:927150/1‑71 (MQ=255) aGCGAGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGcccc > 1:503442/1‑71 (MQ=255) gCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGcccc < 1:888011/70‑1 (MQ=255) gTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGt < 1:2193253/71‑1 (MQ=255) tGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGt < 1:2036432/70‑1 (MQ=255) gCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGa > 1:740913/1‑71 (MQ=255) ccATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGAc < 1:219601/71‑1 (MQ=255) gTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAGCCAGCCAGCCCCCGTGACCACgg > 1:75508/1‑71 (MQ=255) ttACCGTACTGTGTGCATTCGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACgg < 1:77254/70‑1 (MQ=255) ccGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTAtt < 1:924777/71‑1 (MQ=255) cGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTg < 1:1919451/59‑1 (MQ=255) cTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCACCCAGCTAGCCACCGTGACCACGGTATTTTCgg > 1:1504816/1‑71 (MQ=255) cTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:756330/66‑1 (MQ=255) tgtgtgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGt < 1:2042167/53‑1 (MQ=255) tgtgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGct < 1:489336/70‑1 (MQ=255) tgtgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGc < 1:866077/69‑1 (MQ=255) aTTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtttt < 1:40310/60‑1 (MQ=255) aTTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtttt < 1:1485545/60‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGAc < 1:2145835/48‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTc < 1:1837104/60‑1 (MQ=255) gATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCtt > 1:2059271/1‑71 (MQ=255) ccACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTcaac < 1:352193/71‑1 (MQ=255) ccGGTTGCCACTTTCAACCAGCCAGCCCCCGTGAACACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAAt < 1:1503538/71‑1 (MQ=255) cGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:1141735/71‑1 (MQ=255) ccACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCaa < 1:1798116/67‑1 (MQ=255) ccACTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTc < 1:1743103/70‑1 (MQ=255) ccACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTc < 1:925347/70‑1 (MQ=255) ttCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt > 1:1335621/1‑70 (MQ=255) ttCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt > 1:2134004/1‑70 (MQ=255) aaCCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGt < 1:1547721/71‑1 (MQ=255) aCCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTa < 1:409790/71‑1 (MQ=255) aCCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTa < 1:152866/71‑1 (MQ=255) | CGATAGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTA > minE/1073603‑1073735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |