| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,081,858 | T→C | 27.3% | N104S (AAC→AGC) | ydhY ← | predicted 4Fe‑4S ferridoxin‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,081,858 | 0 | T | C | 27.3% | 65.3 / 30.8 | 44 | N104S (AAC→AGC) | ydhY | predicted 4Fe‑4S ferridoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base T (13/19); new base C (4/8); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
TGCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCGTTGA > minE/1081790‑1081927 | tGCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTg > 1:1321332/1‑71 (MQ=255)tGCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTg > 1:49483/1‑71 (MQ=255) gCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTg < 1:670073/70‑1 (MQ=255) ggTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAaat < 1:1137488/71‑1 (MQ=255) ggTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaat < 1:1016005/71‑1 (MQ=255) cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGc < 1:1611726/71‑1 (MQ=255) cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc < 1:911584/71‑1 (MQ=255) gcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaat < 1:809556/64‑1 (MQ=255) gcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaat < 1:617688/64‑1 (MQ=255) gTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCa < 1:1052196/54‑1 (MQ=255) tataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATgg < 1:1410366/71‑1 (MQ=255) tataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATg > 1:1602271/1‑70 (MQ=255) tataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaat < 1:792552/60‑1 (MQ=255) tataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATg < 1:1450989/70‑1 (MQ=255) taGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa > 1:903254/1‑60 (MQ=255) tGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAaataa > 1:1015650/1‑56 (MQ=255) gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGaaa > 1:306690/1‑52 (MQ=255) gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAaata > 1:207000/1‑54 (MQ=255) gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGa > 1:639075/1‑71 (MQ=255) gagaTAGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGa > 1:1222516/1‑71 (MQ=255) agaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAaataa < 1:1374717/54‑1 (MQ=255) agaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAaataa < 1:2084098/54‑1 (MQ=255) aTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATAc < 1:660571/71‑1 (MQ=255) aTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATAc < 1:2204201/71‑1 (MQ=255) cATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGga < 1:140056/70‑1 (MQ=255) aTACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGa < 1:1217686/62‑1 (MQ=255) cAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAg > 1:1919031/1‑37 (MQ=255) cAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag > 1:219430/1‑70 (MQ=255) cAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag < 1:906491/70‑1 (MQ=255) cAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag < 1:1112744/70‑1 (MQ=255) ccgccgccAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGt < 1:561937/71‑1 (MQ=255) tccgccAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGa < 1:1569827/52‑1 (MQ=255) cgccAGAGCCAACCGCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTccc > 1:408890/1‑70 (MQ=255) cgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTccc < 1:1023141/70‑1 (MQ=255) agCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAg > 1:195838/1‑71 (MQ=255) gCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGc > 1:1441972/1‑71 (MQ=255) gCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGc > 1:1990404/1‑71 (MQ=255) gCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAATGAGc < 1:1314607/71‑1 (MQ=255) ccccGTTGTCGCCAAAGAAATAATTGCGATGGAtttt < 1:2205584/37‑1 (MQ=255) ccccGTTGTCGCCAAAGAAATAATTGCGATGGAtttt < 1:709408/37‑1 (MQ=255) ccccGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGa < 1:1904312/39‑1 (MQ=255) cccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATa > 1:1563508/1‑40 (MQ=255) ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCAt > 1:31428/1‑67 (MQ=255) gTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCGTTGa < 1:1100445/71‑1 (MQ=255) | TGCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCGTTGA > minE/1081790‑1081927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |