| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,094,567 | T→C | 29.2% | I604V (ATT→GTT) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,094,567 | 0 | T | C | 29.2% | 30.1 / 16.2 | 24 | I604V (ATT→GTT) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (9/8); new base C (4/3); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
CAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCG > minE/1094498‑1094634 | cAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATc > 1:1133974/1‑71 (MQ=255)cAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATc > 1:462944/1‑71 (MQ=255) cgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTg < 1:929772/71‑1 (MQ=255) gATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTgg < 1:1483033/71‑1 (MQ=255) gATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTgg < 1:293045/71‑1 (MQ=255) cGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTg < 1:625535/70‑1 (MQ=255) caaACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCa > 1:1475719/1‑71 (MQ=255) tcGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTg < 1:1498574/60‑1 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:682833/1‑71 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:1140323/1‑71 (MQ=255) aCCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGAc > 1:2058383/1‑70 (MQ=255) cAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTAc < 1:1294303/71‑1 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATc < 1:451907/51‑1 (MQ=255) gCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg < 1:645599/70‑1 (MQ=255) gCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACgg > 1:1775746/1‑71 (MQ=255) gCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACgg > 1:1778249/1‑71 (MQ=255) gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCAt > 1:2164411/1‑70 (MQ=255) gcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaa < 1:585193/71‑1 (MQ=255) cgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaa < 1:1071617/70‑1 (MQ=255) cTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:364101/71‑1 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGCCGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:804656/1‑71 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAg > 1:2064512/1‑65 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:1766051/1‑71 (MQ=255) tCGGTGAATGAATCCGGATCTGGGTGATCTTCATCg > 1:519268/1‑36 (MQ=255) tgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGAc > 1:439743/1‑43 (MQ=255) gaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCg > 1:1943800/1‑71 (MQ=255) | CAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCG > minE/1094498‑1094634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |