| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,097,909 | T→C | 37.5% | intergenic (+32/‑397) | ydiK → / → ydiL | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,097,909 | 0 | T | C | 37.5% | 15.9 / 22.7 | 24 | intergenic (+32/‑397) | ydiK/ydiL | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (9/6); new base C (4/5); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGT > minE/1097839‑1097979 | ctcGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGt > 1:1992355/1‑71 (MQ=255) ggCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCt > 1:646773/1‑70 (MQ=255) aTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGat < 1:845856/71‑1 (MQ=255) tCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAac < 1:1924486/56‑1 (MQ=255) cGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGata < 1:1915246/62‑1 (MQ=255) cGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCt < 1:1006657/54‑1 (MQ=255) aaTAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGATaa > 1:947467/1‑54 (MQ=255) aTAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctct > 1:1222076/1‑71 (MQ=255) tAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCtt < 1:1444061/71‑1 (MQ=255) ttCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatat > 1:2156794/1‑71 (MQ=255) atcaGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt < 1:397356/68‑1 (MQ=255) gcggcTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCtt < 1:1371706/71‑1 (MQ=255) cggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTa > 1:1876153/1‑71 (MQ=255) tctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGc > 1:350302/1‑71 (MQ=255) tGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCa > 1:1044463/1‑71 (MQ=255) cgccTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTcc < 1:1099315/56‑1 (MQ=255) ccTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAg > 1:253851/1‑71 (MQ=255) ccTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAg > 1:2055154/1‑71 (MQ=255) tGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt < 1:844493/49‑1 (MQ=255) tGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt < 1:880120/49‑1 (MQ=255) gATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAg < 1:348267/68‑1 (MQ=255) aTTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTgg > 1:687346/1‑71 (MQ=255) aTTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTgg > 1:1648764/1‑71 (MQ=255) gTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTgg > 1:1849663/1‑68 (MQ=255) gTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgtag > 1:2005487/1‑71 (MQ=255) ttAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgtagt < 1:2186606/71‑1 (MQ=255) | CTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGT > minE/1097839‑1097979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |