| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 144,272 | C→A | 38.4% | G194V (GGC→GTC) | yadB ← | glutamyl‑Q tRNA(Asp) synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 144,272 | 0 | C | A | 38.4% | 75.6 / 28.9 | 44 | G194V (GGC→GTC) | yadB | glutamyl‑Q tRNA(Asp) synthetase |
| Reads supporting (aligned to +/- strand): ref base C (14/13); new base A (13/4); total (27/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGATGAATGA > minE/144202‑144338 | aaGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGc > 1:914356/1‑71 (MQ=255) cAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGaaa < 1:270419/70‑1 (MQ=255) cAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAAt > 1:724739/1‑71 (MQ=255) cAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAAt > 1:685726/1‑71 (MQ=255) cAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAAt > 1:1495865/1‑71 (MQ=255) cGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGat < 1:1568508/71‑1 (MQ=255) tttGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGAtcatca < 1:1880090/71‑1 (MQ=255) tttGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGAtcatca < 1:1397482/71‑1 (MQ=255) cTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACcac < 1:1423260/71‑1 (MQ=255) ttACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACcac > 1:1104902/1‑70 (MQ=255) tGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGa > 1:1240066/1‑52 (MQ=255) ttCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGa < 1:1034039/44‑1 (MQ=255) cAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTg > 1:1037392/1‑71 (MQ=255) aaTCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTg < 1:1440225/70‑1 (MQ=255) aaTCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTg < 1:1809753/70‑1 (MQ=255) aaTCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGt < 1:42635/71‑1 (MQ=255) aaTCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGt < 1:828852/71‑1 (MQ=255) aaTCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGt < 1:2041978/71‑1 (MQ=255) aaTCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGt < 1:997840/71‑1 (MQ=255) atcagatcagCCCCACGCACTATTTCTGTAACGCCCTGGAAATGa > 1:1040494/1‑45 (MQ=255) atcagatcagCCCCACGCACTATTTCTGTAACGCCCTGGAAATGa > 1:1367968/1‑45 (MQ=255) atcagatcagCCCCACGCACTATTTCAGTAACGCCCTgg > 1:1605957/1‑39 (MQ=255) tcagatcagCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACcac < 1:1420787/55‑1 (MQ=255) gatcagCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc > 1:2011363/1‑70 (MQ=255) tcagCCCCACGCACTATTTCTGTAACGCCCTGGAAATGat > 1:623477/1‑40 (MQ=255) cagCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGaa > 1:462519/1‑70 (MQ=255) cccACGCACTATTTCTGTAACGCCCTGGAAATGatcatc > 1:2111964/1‑39 (MQ=255) cGCACTATTTCTGTAACGCCCTGGAAATGAtcatca > 1:2173082/1‑36 (MQ=255) gCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCa > 1:2207124/1‑71 (MQ=255) cACTATTTCTGTAACGACCTGGAAATGATCATCAAc > 1:777690/1‑36 (MQ=255) aTTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGcc < 1:374996/42‑1 (MQ=255) aTTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTa < 1:459181/50‑1 (MQ=255) aTTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGc > 1:1827107/1‑70 (MQ=255) aTTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAg > 1:1619918/1‑51 (MQ=255) tttCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGa > 1:367086/1‑71 (MQ=255) tttCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGa > 1:1760948/1‑71 (MQ=255) tttCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACGACCCATCACGGCGa > 1:1702878/1‑71 (MQ=255) tttCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGc < 1:1125117/69‑1 (MQ=255) ttCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTa < 1:1759832/48‑1 (MQ=255) tCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCa > 1:1638767/1‑60 (MQ=255) tCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGaacaa > 1:1332407/1‑56 (MQ=255) gTAACGACCTGGAAATGATCATCAACCACACCAGCCAgg > 1:427528/1‑39 (MQ=37) gTAACGACCTGGAAATGATCATCAACCACAACAGCCAgg > 1:1815935/1‑39 (MQ=255) gTAACGACCTGGAAATGATCATCAACCACAACAGCCAgg > 1:910196/1‑39 (MQ=255) aCGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGatgaatga > 1:145412/1‑70 (MQ=255) | AAGCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGATGAATGA > minE/144202‑144338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |