| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,099,149 | A→G | 35.3% | T130A (ACA→GCA) | ydiM → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,099,149 | 0 | A | G | 35.3% | 30.1 / 31.6 | 34 | T130A (ACA→GCA) | ydiM | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (8/14); new base G (8/4); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATTAGCCTGTT > minE/1099080‑1099219 | aTGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGa > 1:401463/1‑70 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGc > 1:1574037/1‑71 (MQ=255) cAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAata > 1:486019/1‑71 (MQ=255) tttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGa > 1:1864908/1‑58 (MQ=255) ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATAttt > 1:816728/1‑69 (MQ=255) ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATAtttt < 1:2015904/70‑1 (MQ=255) ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATAtttt > 1:788291/1‑70 (MQ=255) gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTttaatta < 1:1670328/70‑1 (MQ=255) gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTttaat < 1:2015627/68‑1 (MQ=255) cAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCa > 1:1305080/1‑71 (MQ=255) ttATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATAttt < 1:2113900/53‑1 (MQ=255) tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc > 1:1268993/1‑71 (MQ=255) aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGa > 1:1967590/1‑39 (MQ=255) aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGtt > 1:1639268/1‑68 (MQ=255) cccAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATAtttt < 1:1225101/50‑1 (MQ=255) cAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaat < 1:1223115/51‑1 (MQ=255) cAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCg < 1:934314/71‑1 (MQ=255) cAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCg < 1:959988/71‑1 (MQ=255) tGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACaa > 1:972015/1‑71 (MQ=255) gATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACaa < 1:1424589/70‑1 (MQ=255) gATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACaa < 1:1013221/70‑1 (MQ=255) gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAttttt < 1:244531/71‑1 (MQ=255) gAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt < 1:1682098/70‑1 (MQ=255) aaGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCa < 1:640863/47‑1 (MQ=255) gCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCAtt > 1:2023182/1‑47 (MQ=255) gCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAttttt < 1:310566/68‑1 (MQ=255) gCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt < 1:1769502/71‑1 (MQ=255) cTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatta > 1:2077529/1‑71 (MQ=255) ttttCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc > 1:212140/1‑51 (MQ=255) tttCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTAcc > 1:985851/1‑71 (MQ=255) tttCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatta > 1:189786/1‑69 (MQ=255) tCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGa > 1:1578519/1‑55 (MQ=255) aCCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCAtt < 1:1121448/70‑1 (MQ=255) cTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATTAGc < 1:1552936/71‑1 (MQ=255) cTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt < 1:245048/51‑1 (MQ=255) tGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt > 1:1796450/1‑48 (MQ=255) tGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt > 1:1203213/1‑48 (MQ=255) aCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATAATTAGCCTGtt < 1:1840837/71‑1 (MQ=255) | ATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATTAGCCTGTT > minE/1099080‑1099219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |