| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,249,224 | C→T | 27.8% | D17N (GAT→AAT) | ruvB ← | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,249,224 | 0 | C | T | 27.8% | 56.3 / 24.3 | 36 | D17N (GAT→AAT) | ruvB | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Reads supporting (aligned to +/- strand): ref base C (15/11); new base T (7/3); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAACGCGG > minE/1249156‑1249291 | tgAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATc < 1:90374/69‑1 (MQ=255) aacgaacCTGCGGCTGCCCAACATACTCTTCCAGTAATTTGTGGCGAATGGCGCGATCTGCTACATCTTcc > 1:460973/1‑71 (MQ=255) aacgaacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTcc > 1:2087623/1‑71 (MQ=255) cgaacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCgg > 1:237573/1‑71 (MQ=255) aacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCgg > 1:1505757/1‑69 (MQ=255) aacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCa < 1:140990/71‑1 (MQ=255) ccTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCtt < 1:416720/63‑1 (MQ=255) gCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTg > 1:1926654/1‑71 (MQ=255) gCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTg > 1:43541/1‑71 (MQ=255) gCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTg > 1:1623097/1‑71 (MQ=255) cTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGt < 1:1059898/66‑1 (MQ=255) cTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGt < 1:1006042/66‑1 (MQ=255) gACCAACATACTCTTCCAGTAATTTGGGGCGAATGGAGCGATCTGCTACATTTTCCGGCaaa > 1:1576348/1‑62 (MQ=255) aTACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGaa < 1:936/70‑1 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:934661/1‑71 (MQ=255) tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGaaaa > 1:2155275/1‑51 (MQ=255) ttCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGAc > 1:1606519/1‑71 (MQ=255) tAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTgg > 1:620700/1‑47 (MQ=255) aaTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGt > 1:1242667/1‑67 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCt < 1:215733/71‑1 (MQ=255) aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCt > 1:1300252/1‑71 (MQ=255) tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCt < 1:159766/70‑1 (MQ=255) tGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc > 1:1609622/1‑45 (MQ=255) tGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc > 1:593810/1‑45 (MQ=255) tGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCa > 1:1091011/1‑57 (MQ=255) tGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTc < 1:514025/70‑1 (MQ=255) tGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTc > 1:1948664/1‑70 (MQ=255) gggCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCaa < 1:1296643/70‑1 (MQ=255) gggCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAat < 1:387883/71‑1 (MQ=255) tGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCa < 1:11100/48‑1 (MQ=255) tGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCa < 1:1892053/48‑1 (MQ=255) ggCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCtt > 1:1573669/1‑59 (MQ=255) aTCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAatca > 1:809172/1‑59 (MQ=255) aCATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACgg > 1:419323/1‑39 (MQ=255) cATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAAcgc < 1:1143311/69‑1 (MQ=255) cATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAAcgc < 1:244801/69‑1 (MQ=255) cATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAACgcgg > 1:751968/1‑71 (MQ=255) | TGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAACGCGG > minE/1249156‑1249291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |