| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,252,050 | T→C | 26.9% | K6K (AAA→AAG) | yebC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,252,050 | 0 | T | C | 26.9% | 36.7 / 15.9 | 26 | K6K (AAA→AAG) | yebC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (13/6); new base C (4/3); total (17/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01 | |||||||||||
ATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAA > minE/1251985‑1252115 | aTTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTAc > 1:244640/1‑70 (MQ=255) ttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTAt < 1:784158/70‑1 (MQ=255) tAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATg < 1:2144073/70‑1 (MQ=255) tttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATa > 1:698812/1‑70 (MQ=255) tttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGAcc > 1:889206/1‑63 (MQ=255) tttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGAcc > 1:758892/1‑63 (MQ=255) ttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTAt > 1:1964868/1‑58 (MQ=255) ttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTTcc > 1:556770/1‑66 (MQ=255) ccGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATaaaaa < 1:311125/70‑1 (MQ=255) cgcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCAtt < 1:871938/46‑1 (MQ=255) cgcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCAtt < 1:1345735/46‑1 (MQ=255) cgcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCAtt < 1:1027202/46‑1 (MQ=255) aGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCa < 1:1798935/70‑1 (MQ=255) cATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGa > 1:97327/1‑47 (MQ=255) cATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGa > 1:2073121/1‑47 (MQ=255) tCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAg < 1:1984996/71‑1 (MQ=255) tGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTa > 1:1711222/1‑39 (MQ=255) tGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAatat > 1:411378/1‑58 (MQ=255) gcgcAGCTTTACGATGTCTGGTGTTGGCCCATTTAc > 1:1146880/1‑36 (MQ=255) cgcAGCTTTACGATGTCTGGTGTTGGCCCATTTAc < 1:288584/35‑1 (MQ=255) cgcAGCTTTACGATGTCTGGTGTTGGCCCATTTAc < 1:1545861/35‑1 (MQ=255) ttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCcctgcc > 1:1108965/1‑66 (MQ=255) ttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCcctgcc > 1:930535/1‑66 (MQ=255) ttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGtt > 1:1126677/1‑70 (MQ=255) ttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGtt > 1:1064634/1‑70 (MQ=255) tCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATAtctc < 1:1731237/44‑1 (MQ=255) tCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAg < 1:409592/51‑1 (MQ=255) gtTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAAt > 1:1162434/1‑70 (MQ=255) ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaa > 1:769913/1‑69 (MQ=255) tttACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTc < 1:2185148/48‑1 (MQ=255) tttACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGc < 1:978950/52‑1 (MQ=255) | ATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAA > minE/1251985‑1252115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |