| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,257,376 | T→C | 26.9% | F7L (TTT→CTT) | yecP → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,257,376 | 0 | T | C | 26.9% | 80.5 / 36.8 | 52 | F7L (TTT→CTT) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (18/20); new base C (5/9); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
CCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCT > minE/1257307‑1257441 | ccAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACt < 1:1413934/70‑1 (MQ=255) cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:997033/71‑1 (MQ=255) cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:681098/71‑1 (MQ=255) aGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACctt > 1:223717/1‑70 (MQ=255) gCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt > 1:336635/1‑70 (MQ=255) cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtc < 1:1531343/71‑1 (MQ=255) cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATAGACTTTGGTAACCTTTAtt < 1:1880113/70‑1 (MQ=255) tAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTctct > 1:1956749/1‑71 (MQ=255) aaCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTctct < 1:1247256/70‑1 (MQ=255) aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGa < 1:451966/71‑1 (MQ=255) aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGa < 1:1689455/71‑1 (MQ=255) tttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGa > 1:609898/1‑69 (MQ=255) ttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTg > 1:1191614/1‑71 (MQ=255) gTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGcc < 1:1726217/70‑1 (MQ=255) gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatca < 1:770188/70‑1 (MQ=255) gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatca < 1:1960892/70‑1 (MQ=255) cATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1316987/71‑1 (MQ=255) ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATtct < 1:822858/47‑1 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTa > 1:1453519/1‑41 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTgaca > 1:1937168/1‑71 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:294509/1‑71 (MQ=255) aaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt > 1:435537/1‑41 (MQ=255) aaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt > 1:1309312/1‑41 (MQ=255) aaaGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:1825870/1‑68 (MQ=255) gCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:1335592/35‑1 (MQ=255) ggACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:163534/71‑1 (MQ=255) gACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:1144161/70‑1 (MQ=255) gACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTg < 1:195771/38‑1 (MQ=255) gACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa > 1:1059900/1‑70 (MQ=255) gCTGCATGATCGACTTTTGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACg < 1:1985465/71‑1 (MQ=255) gCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACg < 1:695214/71‑1 (MQ=255) gCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACg < 1:473453/71‑1 (MQ=255) tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaa < 1:1448476/41‑1 (MQ=255) tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaa < 1:316927/41‑1 (MQ=255) cATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGc > 1:1331755/1‑71 (MQ=255) aTGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGcc > 1:1258800/1‑71 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatc < 1:783556/42‑1 (MQ=255) tGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAatc > 1:1440982/1‑42 (MQ=255) tGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAatc > 1:341449/1‑42 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACg > 1:1270333/1‑63 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACg > 1:400264/1‑63 (MQ=255) tCGACTATGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgcg > 1:283351/1‑71 (MQ=255) cGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:396451/1‑46 (MQ=255) gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:1304099/71‑1 (MQ=255) gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:263943/71‑1 (MQ=255) gACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:1397412/71‑1 (MQ=255) aCTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1767500/42‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcac < 1:1564928/43‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcac < 1:1087574/43‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcac < 1:1370577/43‑1 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCacac > 1:555171/1‑43 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCacac > 1:117504/1‑43 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa > 1:2094162/1‑66 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa > 1:2091597/1‑66 (MQ=255) tGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGAt < 1:31816/69‑1 (MQ=255) gTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:1051596/71‑1 (MQ=255) gTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:522446/71‑1 (MQ=255) | CCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCT > minE/1257307‑1257441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |