| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,260,748 | G→A | 9.7% | S59L (TCA→TTA) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,260,748 | 0 | G | A | 9.7% | 115.7 / 4.1 | 41 | S59L (TCA→TTA) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/22); new base A (2/2); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TGTGTACCTGATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACC > minE/1260683‑1260818 | tgtgtACCTGATCCGCCGCCGTAGACTGTAAGGAATTCGGTATTGTTTTCGCCAGCGCGCCTGTTgaagaa < 1:1659084/71‑1 (MQ=255)tgtgtACCTGATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTgaagaa < 1:2159942/71‑1 (MQ=255) tACCTGATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACa < 1:1653838/70‑1 (MQ=255) ccTGATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAAt < 1:803287/70‑1 (MQ=255) cTGATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCt < 1:1296703/71‑1 (MQ=255) gATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTg > 1:1343445/1‑71 (MQ=255) gATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTg > 1:673566/1‑71 (MQ=255) aTCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCtt < 1:1080835/69‑1 (MQ=255) aTCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCtt < 1:1437612/69‑1 (MQ=255) aTCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGc < 1:863163/71‑1 (MQ=255) gccgccgTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTc < 1:2037837/71‑1 (MQ=255) cgccgTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCt < 1:1329605/71‑1 (MQ=255) gTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAAGAATCTTGCCGTCCtt < 1:1288142/68‑1 (MQ=255) tAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACt < 1:129701/71‑1 (MQ=255) tGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTa > 1:1426765/1‑70 (MQ=255) tGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTAc > 1:2013647/1‑71 (MQ=255) tAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACaa < 1:1590217/47‑1 (MQ=255) tAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACaa < 1:1348259/47‑1 (MQ=255) tAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACaa < 1:1128939/47‑1 (MQ=255) tAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGt > 1:172541/1‑71 (MQ=255) aGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGtt > 1:549128/1‑70 (MQ=255) ggAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCa < 1:1547844/71‑1 (MQ=255) aTTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTc < 1:26751/51‑1 (MQ=255) ttCGGTATGGTTTTCGCCAGCGCGCCTGTTgaaga < 1:901420/35‑1 (MQ=255) ttCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACa < 1:773835/39‑1 (MQ=255) ttCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCg < 1:1315889/48‑1 (MQ=255) cGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGaaa > 1:1794627/1‑35 (MQ=255) aTGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCa > 1:2193782/1‑61 (MQ=255) aTGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCa > 1:994084/1‑61 (MQ=255) aTGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTcccc > 1:1990284/1‑70 (MQ=255) aTGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTcccc > 1:1901704/1‑70 (MQ=255) tttCGCCAGCGCGCCTGTTGAAGAAACAATCTTGc < 1:1645690/35‑1 (MQ=255) ttCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATc > 1:1829246/1‑57 (MQ=255) gCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCcgc < 1:1961687/71‑1 (MQ=255) gCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCcgc < 1:289399/71‑1 (MQ=255) gCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCcg < 1:943327/70‑1 (MQ=255) ccAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCcgcg > 1:537066/1‑71 (MQ=255) tGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGAttt > 1:285055/1‑71 (MQ=255) tGTTAAAGAAACAATCTTGCCGTCCTTCACTTCTa > 1:1604748/1‑35 (MQ=255) tGTTAAAGAAACAATCTTGCCGTCCTTCACTTCTAc > 1:2092778/1‑36 (MQ=255) tGTTAAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCt < 1:1233119/46‑1 (MQ=255) tGTTAAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCt < 1:578193/46‑1 (MQ=255) ttGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGAtttt < 1:131927/70‑1 (MQ=255) ttGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTa > 1:984904/1‑71 (MQ=255) gaagaaACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGAt < 1:1393888/65‑1 (MQ=255) gaagaaACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTa > 1:90284/1‑69 (MQ=255) gaagaaACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTAcc > 1:718415/1‑71 (MQ=255) gaagaaACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTAcc > 1:1860410/1‑71 (MQ=255) gaagaaACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTAc < 1:1271956/70‑1 (MQ=255) gaagaaACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTAc < 1:210535/70‑1 (MQ=255) | TGTGTACCTGATCCGCCGCCGTAGACTGTAAGGAATTCGGTATGGTTTTCGCCAGCGCGCCTGTTGAAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACC > minE/1260683‑1260818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |