| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,291,126 | G→A | 16.3% | intergenic (+241/+212) | yeeN → / ← asnW | conserved hypothetical protein/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,291,126 | 0 | G | A | 16.3% | 125.4 / 14.9 | 49 | intergenic (+241/+212) | yeeN/asnW | conserved hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base G (18/23); new base A (2/6); total (20/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGA > minE/1291059‑1291187 | tACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTAc > 1:1090032/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTtata > 1:510611/1‑69 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACg > 1:2060597/1‑71 (MQ=255) tAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTAc < 1:21546/70‑1 (MQ=255) tAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTAc > 1:32409/1‑70 (MQ=255) gTTTCCCGATAAAGACGCTATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt > 1:1810901/1‑70 (MQ=255) gTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt < 1:2041757/70‑1 (MQ=255) gTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt > 1:1736001/1‑70 (MQ=255) tttCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCa > 1:2212776/1‑71 (MQ=255) aTAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACa < 1:1265837/71‑1 (MQ=255) gATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTAc < 1:115715/45‑1 (MQ=255) gATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTAc < 1:1865195/45‑1 (MQ=255) gATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGt < 1:1019417/48‑1 (MQ=255) tAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg > 1:1201942/1‑45 (MQ=255) cGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTg < 1:509118/65‑1 (MQ=255) aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg < 1:842077/38‑1 (MQ=255) aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg < 1:1899646/38‑1 (MQ=255) aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg < 1:2024211/38‑1 (MQ=255) aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGaa < 1:743328/71‑1 (MQ=255) aTCCCGCATAAGGAATTTTTCTCTTTATCTTTGTACgg < 1:1506422/38‑1 (MQ=255) tCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCtt < 1:2071340/65‑1 (MQ=255) tCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGaa < 1:454761/49‑1 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGAcc > 1:1858906/1‑61 (MQ=255) cGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt > 1:422522/1‑71 (MQ=255) cGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:1870236/71‑1 (MQ=255) cATAAGTCATTTTTCTCTTTATCTTTGTACGGTACTTCa < 1:347321/39‑1 (MQ=255) cATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt < 1:1322790/37‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGaa > 1:1308440/1‑42 (MQ=255) aaGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:1960951/1‑68 (MQ=255) aGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTc < 1:931697/71‑1 (MQ=255) gCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTg < 1:1951452/71‑1 (MQ=255) cATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGt > 1:506842/1‑71 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:1539108/60‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGt < 1:2086261/69‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg < 1:840186/71‑1 (MQ=255) tttttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGtt < 1:2075641/70‑1 (MQ=255) ttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:365737/59‑1 (MQ=255) ttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:189591/59‑1 (MQ=255) ttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg < 1:266453/70‑1 (MQ=255) ttttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg > 1:731967/1‑71 (MQ=255) ttCTCTTTATCTTTGTACGTTACTTCATGGAACAGAGttt > 1:489183/1‑40 (MQ=255) ttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:1364190/1‑59 (MQ=255) tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGa > 1:512693/1‑39 (MQ=255) tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGgggaggga > 1:1931186/1‑71 (MQ=255) tttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGgggagg < 1:1325900/69‑1 (MQ=255) tATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg < 1:600280/63‑1 (MQ=255) tATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg > 1:730115/1‑61 (MQ=255) tCTTTGTACGGTACTTCATGGAACAGAGTTTTTGCCCTTGCGAATCGTGATGTCTGTTGggga > 1:1933247/1‑63 (MQ=255) tCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGggga > 1:533573/1‑63 (MQ=255) tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGt < 1:159227/51‑1 (MQ=255) | TACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGA > minE/1291059‑1291187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |