| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,418,334 | G→A | 23.8% | V95I (GTC→ATC) | narP → | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,418,334 | 0 | G | A | 23.8% | 81.1 / 22.6 | 42 | V95I (GTC→ATC) | narP | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Reads supporting (aligned to +/- strand): ref base G (15/17); new base A (2/8); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01 | |||||||||||
ATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTGCTGG > minE/1418266‑1418403 | aTGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTc > 1:360774/1‑71 (MQ=255)aTGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTc > 1:929314/1‑71 (MQ=255) tGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCt < 1:114017/71‑1 (MQ=255) ccTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCTATGCCTCCAGCGATGTCtt > 1:510995/1‑70 (MQ=255) ccTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCtt < 1:1963660/70‑1 (MQ=255) ccTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCtt > 1:1602486/1‑70 (MQ=255) ccTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCtt < 1:1544162/70‑1 (MQ=255) tGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCttt > 1:2142081/1‑68 (MQ=255) cgcAGGTATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCt < 1:998006/71‑1 (MQ=255) cgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCt < 1:1085541/71‑1 (MQ=255) gggATGGCGTTACCTCGCAAATTATTATCATGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTgt > 1:383472/1‑68 (MQ=255) gggATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGa > 1:890314/1‑69 (MQ=255) gggATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa < 1:1652041/71‑1 (MQ=255) gATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATg > 1:353256/1‑55 (MQ=255) gATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGAGATg > 1:622624/1‑55 (MQ=255) ttACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGt < 1:386505/49‑1 (MQ=255) tACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGt < 1:1203313/48‑1 (MQ=255) tACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgc < 1:292461/70‑1 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGgcg > 1:1074081/1‑70 (MQ=255) ccGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCa < 1:435796/71‑1 (MQ=255) ccGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCa < 1:1562304/71‑1 (MQ=255) ccGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCa < 1:505867/71‑1 (MQ=255) ccGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCa < 1:810108/71‑1 (MQ=255) ccGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCa < 1:2070515/71‑1 (MQ=255) attattATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGt > 1:445529/1‑69 (MQ=255) tattatCCTGACCGTATCCGATGCCTCCAGCGATGt < 1:481336/36‑1 (MQ=255) tattatCCTGACCGTATCCGATGCCTCCAGCGATGt < 1:2098322/36‑1 (MQ=255) tattatCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa < 1:1603150/49‑1 (MQ=255) atCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGt > 1:881412/1‑70 (MQ=255) cTGACCGTATACGATGCCTCCAGCGATGTCTTTGCGCTGATAGaa > 1:1962298/1‑44 (MQ=255) aCCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa < 1:1936281/69‑1 (MQ=255) aCCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAG‑‑GCAGACGGTTATCTGTTGAAAGAc < 1:1981095/70‑1 (MQ=255) ccGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGAc < 1:916907/71‑1 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:1996174/1‑71 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:870251/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:1401534/1‑71 (MQ=255) cTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa < 1:2049019/55‑1 (MQ=255) cTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa < 1:1764144/55‑1 (MQ=255) cAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa > 1:950455/1‑54 (MQ=255) cAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa > 1:1661268/1‑54 (MQ=255) cAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCg < 1:1292934/59‑1 (MQ=255) gCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTg < 1:1943164/71‑1 (MQ=255) gCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTg < 1:335887/71‑1 (MQ=255) aTGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTg > 1:179943/1‑44 (MQ=255) gTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaa > 1:356574/1‑44 (MQ=255) gTCTTTGCGCTGATAGAAGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTGCTgg > 1:1075004/1‑70 (MQ=255) | ATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTGCTGG > minE/1418266‑1418403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |