| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 163,666 | C→T | 25.9% | E77K (GAA→AAA) | yaeI ← | predicted phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 163,666 | 0 | C | T | 25.9% | 45.5 / 14.5 | 27 | E77K (GAA→AAA) | yaeI | predicted phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (5/15); new base T (2/5); total (7/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
GCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATC > minE/163596‑163729 | gCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc < 1:995066/71‑1 (MQ=255)gCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc < 1:321299/71‑1 (MQ=255) ccGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt < 1:596721/71‑1 (MQ=255) ccGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCt < 1:1006481/71‑1 (MQ=255) aaaTTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTc > 1:764694/1‑65 (MQ=255) aaaTTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGATTTTGTTCTATGcc < 1:1726775/71‑1 (MQ=255) tCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATc < 1:342461/66‑1 (MQ=255) tCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGt > 1:1314379/1‑70 (MQ=255) taataCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGaaa < 1:249385/71‑1 (MQ=255) ataCGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAAt < 1:968233/70‑1 (MQ=255) tCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTa > 1:283303/1‑70 (MQ=255) cAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACaa < 1:531981/71‑1 (MQ=255) cAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACaa < 1:43173/71‑1 (MQ=255) cAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACaa < 1:1504962/71‑1 (MQ=255) aGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTaa < 1:963241/62‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAATAGCAATCGCGTCAGAAATCa < 1:1174133/48‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTa < 1:2173239/54‑1 (MQ=255) tCAAATCGGGCTTTTGTTTTATGCCAAGAGCAATCGCGTAAGGAATCAGGCTTAAAGGaa > 1:2160551/1‑60 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAgg < 1:229342/50‑1 (MQ=255) tCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAgg < 1:1514360/50‑1 (MQ=255) aaaTCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGt < 1:345412/70‑1 (MQ=255) aaaTCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGt < 1:1615543/70‑1 (MQ=255) aaaTCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGt < 1:97447/70‑1 (MQ=255) aaaTCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTa < 1:1037005/71‑1 (MQ=255) cGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATg > 1:1780560/1‑70 (MQ=255) gggCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGaaa < 1:65231/38‑1 (MQ=255) tttGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACgaga > 1:2014627/1‑58 (MQ=255) tttGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACgaga > 1:1889174/1‑58 (MQ=255) ttGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGga > 1:1922242/1‑65 (MQ=255) ttGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATc < 1:1485207/69‑1 (MQ=255) | GCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAAGAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATC > minE/163596‑163729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |