| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,475,665 | A→T | 20.7% | T244S (ACC→TCC) | nrdB → | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,475,665 | 0 | A | T | 20.7% | 46.7 / 7.3 | 24 | T244S (ACC→TCC) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base A (10/9); new base T (2/3); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AGAACGCGAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATT > minE/1475595‑1475730 | aGAACGCGAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGa > 1:227064/1‑71 (MQ=255) gATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAg > 1:1376360/1‑70 (MQ=255) aTGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCa < 1:1460842/71‑1 (MQ=255) tGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCat < 1:46787/71‑1 (MQ=255) gAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCata < 1:37548/70‑1 (MQ=255) aaGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATg < 1:253029/71‑1 (MQ=255) ggCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc < 1:1811072/70‑1 (MQ=255) aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCt > 1:1743559/1‑67 (MQ=255) attattCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCtgctgct > 1:1987174/1‑70 (MQ=255) ttattCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCAc > 1:559007/1‑46 (MQ=255) ttattCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTgcg > 1:198807/1‑70 (MQ=255) tattCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTgcg > 1:1199465/1‑69 (MQ=255) attCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCAccc > 1:416072/1‑46 (MQ=255) ttCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:570890/70‑1 (MQ=255) ttCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:896178/70‑1 (MQ=255) ttGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATctg < 1:1732539/53‑1 (MQ=255) ttGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATctg < 1:906883/53‑1 (MQ=255) cccGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACg < 1:114863/69‑1 (MQ=255) cgaAGCCCTGCACCTGTCCGGCACCCAGCATATGc < 1:936692/35‑1 (MQ=255) gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGAt > 1:1534977/1‑71 (MQ=255) gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGAt > 1:742168/1‑71 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaaa > 1:52975/1‑71 (MQ=255) aCCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAtt > 1:1222518/1‑71 (MQ=255) ccTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaa < 1:358460/67‑1 (MQ=255) gACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTgaga > 1:2162547/1‑56 (MQ=255) | AGAACGCGAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATT > minE/1475595‑1475730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |