Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,485,005 C→T 13.1% D279N (GAT→AAT)  menF ← isochorismate synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,485,0050CT13.1% 100.7 / 7.2 38D279N (GAT→AAT) menFisochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (13/20);  new base T (3/2);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGC  >  minE/1484940‑1485074
                                                                 |                                                                     
ggTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTtattat                                                                     <  1:1351407/68‑1 (MQ=255)
         cTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGc                                                          >  1:820413/1‑70 (MQ=255)
            tAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATTCGCCAt                                                              <  1:1045172/63‑1 (MQ=255)
            tAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                                        >  1:2045107/1‑69 (MQ=255)
            tAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                                        <  1:1474725/69‑1 (MQ=255)
            tAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCAct                                                      >  1:992015/1‑71 (MQ=255)
            tAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCAc                                                       <  1:666113/70‑1 (MQ=255)
             aaTCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCActc                                                     >  1:457612/1‑71 (MQ=255)
              aTCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATTATCCGCCATCAGCCActc                                                     <  1:1764704/70‑1 (MQ=255)
              aTCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCActct                                                    >  1:1038624/1‑71 (MQ=255)
                 gTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCAt                                                              >  1:526381/1‑58 (MQ=255)
                 gTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                                        >  1:322922/1‑64 (MQ=255)
                 gTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTcc                                                  >  1:1032334/1‑70 (MQ=255)
                        gATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAActgct                                          <  1:295885/71‑1 (MQ=255)
                         atatCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATTATCCGCCATCAGCCACTCTCCTAActgct                                          >  1:1397018/1‑70 (MQ=255)
                             cTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCActc                                                     <  1:2032708/55‑1 (MQ=255)
                              ttCCACCACCAGCATGTTCTCGCGCTGGTTTTTATTATCCGCCATCAGCCACTCTCCTAACTGCTgcgc                                      >  1:256430/1‑69 (MQ=255)
                              ttCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCa                                                            <  1:867868/47‑1 (MQ=255)
                              ttCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCa                                                            <  1:2128701/47‑1 (MQ=255)
                                 caccaccaGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCt                                 <  1:1437190/71‑1 (MQ=255)
                                  accaccaGCATGTTCTCGCGCTGGTTTTTATTATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTg                                   <  1:188863/68‑1 (MQ=255)
                                  accaccaGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCtt                                <  1:1701656/71‑1 (MQ=255)
                                   ccaccaGCATGTTCTCGCGCTGGTTTTTATTATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTa                               >  1:834201/1‑71 (MQ=255)
                                     accaGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTatc                             <  1:1439075/71‑1 (MQ=255)
                                      ccaGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCtt                                <  1:674696/67‑1 (MQ=255)
                                      ccaGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCtt                                <  1:552990/67‑1 (MQ=255)
                                         gCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                                        >  1:2180111/1‑40 (MQ=255)
                                         gCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCa                                                        >  1:821661/1‑40 (MQ=255)
                                         gCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTatcat                           >  1:2141890/1‑69 (MQ=255)
                                                   gcgcTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTAttt                >  1:2204261/1‑70 (MQ=255)
                                                    cgcTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGc              <  1:37341/71‑1 (MQ=255)
                                                    cgcTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGc              <  1:1356296/71‑1 (MQ=255)
                                                     gcTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGc              <  1:1398842/70‑1 (MQ=255)
                                                         gTTTTTATCATCCGCCATCATCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTa            <  1:1604728/68‑1 (MQ=255)
                                                         gTTTTTATCATCCGCCATCAGCCACTCTCCTAActgctg                                         >  1:666334/1‑39 (MQ=255)
                                                         gTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTa            <  1:1602269/68‑1 (MQ=255)
                                                         gTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTg         <  1:1339384/71‑1 (MQ=255)
                                                         gTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTg         <  1:1269959/71‑1 (MQ=255)
                                                         gTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTg         <  1:1464918/71‑1 (MQ=255)
                                                               atcatcCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTccc    >  1:783844/1‑70 (MQ=255)
                                                               atcatcCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCg   >  1:225522/1‑71 (MQ=255)
                                                                tcatcCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGc  <  1:59986/71‑1 (MQ=255)
                                                                 |                                                                     
GGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCTGGTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGC  >  minE/1484940‑1485074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: