| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,511,849 | T→C | 58.5% | W373R (TGG→CGG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,511,849 | 0 | T | C | 58.5% | 23.7 / 47.3 | 41 | W373R (TGG→CGG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (7/10); new base C (14/10); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGT > minE/1511780‑1511911 | gTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg < 1:1134539/71‑1 (MQ=255) cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATc < 1:905826/71‑1 (MQ=255) cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATc < 1:357536/71‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGa < 1:989728/71‑1 (MQ=255) gAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGc > 1:1108751/1‑71 (MQ=255) aTCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTc > 1:1515521/1‑71 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg < 1:1108309/51‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGaa > 1:1764828/1‑51 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGaa > 1:390607/1‑51 (MQ=255) aagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAtc > 1:567096/2‑53 (MQ=255) gaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa > 1:1622170/1‑48 (MQ=255) gaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGa > 1:345036/1‑56 (MQ=255) aGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAgcg > 1:1301573/1‑69 (MQ=255) cAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGa > 1:615446/1‑62 (MQ=255) cAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGcc < 1:1049995/70‑1 (MQ=255) cAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGcc < 1:340664/70‑1 (MQ=255) ggAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtc < 1:1242886/71‑1 (MQ=255) aTACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCgtcgtc > 1:397299/1‑71 (MQ=255) tACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:339185/50‑1 (MQ=255) tACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:244260/50‑1 (MQ=255) tACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 1:150249/50‑1 (MQ=255) aCGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtct > 1:1799430/1‑70 (MQ=255) cGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg < 1:676223/70‑1 (MQ=255) cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGGCACTTCTGAGCGCAGc > 1:1663248/1‑61 (MQ=255) cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgt > 1:1435808/1‑71 (MQ=255) gTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtc < 1:1762847/71‑1 (MQ=255) tGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCa > 1:1683849/1‑44 (MQ=255) tAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCgtcgtc < 1:1468889/62‑1 (MQ=255) aCTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGc < 1:402149/69‑1 (MQ=255) aCTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCt > 1:492348/1‑71 (MQ=255) aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCt > 1:621203/1‑71 (MQ=255) tACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg > 1:461450/1‑43 (MQ=255) tACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGc > 1:1556781/1‑71 (MQ=255) aCATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAg > 1:1020126/1‑44 (MQ=255) cATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtc < 1:775474/53‑1 (MQ=255) cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg < 1:1468181/70‑1 (MQ=255) tCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtct < 1:1859303/55‑1 (MQ=255) aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgt > 1:107310/1‑55 (MQ=255) aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg < 1:1376931/71‑1 (MQ=255) aCGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGt < 1:1317038/71‑1 (MQ=255) gCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtct > 1:1859785/1‑50 (MQ=255) | GTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGT > minE/1511780‑1511911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |