| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,523,658 | C→T | 48.6% | Q153* (CAA→TAA) | flk → | predicted flagella assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,523,658 | 0 | C | T | 48.6% | 1.6 / 50.3 | 37 | Q153* (CAA→TAA) | flk | predicted flagella assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (12/7); new base T (11/7); total (23/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCAGCTCTCACCGGAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCT > minE/1523589‑1523725 | aaTCAGCTCTCACCGGAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGc < 1:915494/70‑1 (MQ=255)aaTCAGCTCTCACCGGAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGc < 1:512708/70‑1 (MQ=255) tcACCGGAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCAcag > 1:454330/1‑69 (MQ=255) aCCGGAGCAATTAAAAACCATTCTCACCCTGTTGGAGGAAGGGAAGATGGTTATTCCGTAACCAcagcag > 1:320897/1‑70 (MQ=255) aCCGGAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCAcagc < 1:526308/68‑1 (MQ=255) ggAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGc < 1:550291/56‑1 (MQ=255) ggAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAgcgc > 1:1357755/1‑70 (MQ=255) gCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCAGCAACCACAGCAGCGCGAgg < 1:771779/71‑1 (MQ=255) ttAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGc < 1:695435/49‑1 (MQ=255) ttAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGcg < 1:1539270/69‑1 (MQ=255) ttAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGcgac > 1:994006/1‑71 (MQ=255) ttAAAAACAATTCTCACCCTGTAGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGcg < 1:1055413/69‑1 (MQ=255) tAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGcgac > 1:1814360/1‑70 (MQ=255) aagaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGcgacc > 1:948499/4‑70 (MQ=255) aaaaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGcgacc > 1:1852275/1‑70 (MQ=255) aaaaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCATCGCGAGGcgacc > 1:1992110/1‑70 (MQ=255) aaaaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGa > 1:747416/1‑63 (MQ=255) aaaaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGa > 1:315601/1‑63 (MQ=255) aaaaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAgg > 1:523158/1‑65 (MQ=255) aaaaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGcgacc < 1:1253614/70‑1 (MQ=255) aaaaaCCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGc > 1:1679198/1‑66 (MQ=255) aCCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCttt < 1:2188004/68‑1 (MQ=255) aCCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCttt < 1:1919177/68‑1 (MQ=255) aCCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTttat > 1:1823542/1‑70 (MQ=255) ccTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTAc > 1:1175517/1‑71 (MQ=255) ccTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTAc > 1:2035066/1‑71 (MQ=255) tGCAGGAAGGGAAGATGGTTATTCCGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTAc > 1:2132338/1‑66 (MQ=255) tGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGAGCGAGGCGACCGACCGTCCTTTATTACcggcg > 1:1108805/1‑71 (MQ=255) gCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTtta > 1:1856255/1‑61 (MQ=255) gaagggaagATGGTTATTCCGTAACCACAGCAGCGCGAGGCCACCGACCGTCCTTTATTACCGGCGGAGca > 1:421135/1‑71 (MQ=255) aagggaagATGGTTATTCCGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGcac < 1:79231/71‑1 (MQ=255) aagggaagATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGcac < 1:1874337/71‑1 (MQ=255) aagggaagATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGcac < 1:1656218/71‑1 (MQ=255) aagggaagATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGcac < 1:466379/71‑1 (MQ=255) gggaagATGGTTATTCCGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACaa < 1:491673/71‑1 (MQ=255) ggaagATGGTTATTCCGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGcac < 1:1762032/68‑1 (MQ=255) ggTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATgcgc > 1:2166844/1‑68 (MQ=255) ttATTCCGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGcac < 1:1937321/59‑1 (MQ=255) tATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATg > 1:230711/1‑62 (MQ=255) tCCGTAACCACAGCAGCGCGAGGCGACCGACCGTGCTtta > 1:584855/1‑40 (MQ=37) ccGTAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCt > 1:915968/1‑71 (MQ=255) | AATCAGCTCTCACCGGAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCT > minE/1523589‑1523725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |