| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,533,542 | G→A | 29.4% | P51S (CCT→TCT) | prmB ← | N5‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,533,542 | 0 | G | A | 29.4% | 48.6 / 23.9 | 34 | P51S (CCT→TCT) | prmB | N5‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (13/11); new base A (4/6); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
TTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTG > minE/1533476‑1533604 | tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa > 1:1408639/1‑70 (MQ=255)tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa > 1:2172247/1‑70 (MQ=255)tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACaa < 1:1159870/70‑1 (MQ=255)tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACaa < 1:1016429/70‑1 (MQ=255)tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACaa < 1:1782202/70‑1 (MQ=255) cGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAacac < 1:1178256/70‑1 (MQ=255) cGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAacac < 1:483190/70‑1 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTAc > 1:1745192/1‑69 (MQ=255) aCGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa > 1:471289/1‑54 (MQ=255) cgcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGTCTTc < 1:1799575/71‑1 (MQ=255) cgcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTc < 1:475101/71‑1 (MQ=255) gcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCa < 1:1512817/71‑1 (MQ=255) cgcATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACg < 1:1942089/70‑1 (MQ=255) cgcATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACg < 1:147959/70‑1 (MQ=255) cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGt < 1:1676376/52‑1 (MQ=255) atatCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt > 1:711872/1‑70 (MQ=255) atCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCAt < 1:1992154/59‑1 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAacac > 1:156061/1‑41 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc > 1:1550446/1‑71 (MQ=255) tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATc > 1:1151504/1‑71 (MQ=255) cTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa > 1:906347/1‑37 (MQ=255) cTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCg > 1:53271/1‑71 (MQ=255) ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCgg > 1:2075178/1‑71 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc < 1:1842385/67‑1 (MQ=255) atatCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTg < 1:89832/38‑1 (MQ=255) atatCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTg < 1:1419804/71‑1 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:1789057/1‑69 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:363452/71‑1 (MQ=255) aGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGat < 1:2076764/71‑1 (MQ=255) tAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAtt > 1:1550647/1‑71 (MQ=255) agagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:1748046/1‑71 (MQ=255) gagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTg > 1:1711480/1‑71 (MQ=255) cGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:1482442/1‑55 (MQ=255) cGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:807575/1‑55 (MQ=255) | TTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTG > minE/1533476‑1533604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |