| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,572,316 | T→C | 9.3% | *472* (TAA→TGA) | gltX ← | glutamyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,572,316 | 0 | T | C | 9.3% | 107.2 / 4.2 | 43 | *472* (TAA→TGA) | gltX | glutamyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (20/19); new base C (2/2); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCT > minE/1572247‑1572382 | ttGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAAtt < 1:559727/70‑1 (MQ=255) gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATCAct > 1:1061075/1‑71 (MQ=255) gAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct > 1:1307796/1‑71 (MQ=255) aataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATCACTGCTGAtttt < 1:1030235/66‑1 (MQ=255) ataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt > 1:1184102/1‑70 (MQ=255) taaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc < 1:460578/71‑1 (MQ=255) aaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATCACTGCTGATTTTCGCGTTCAGCa < 1:159846/71‑1 (MQ=255) gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa < 1:954328/71‑1 (MQ=255) gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa < 1:443852/71‑1 (MQ=255) gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa < 1:1932366/71‑1 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTa > 1:1039830/1‑39 (MQ=255) attatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgc > 1:1080874/1‑71 (MQ=255) ttatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcg < 1:613531/71‑1 (MQ=255) atCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAg > 1:777740/1‑67 (MQ=255) atCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCt > 1:1270234/1‑71 (MQ=255) cTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc > 1:357707/1‑59 (MQ=255) cTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt < 1:1791316/71‑1 (MQ=255) ccGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa < 1:541194/70‑1 (MQ=255) ccGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa < 1:1815318/70‑1 (MQ=255) ccGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa < 1:2043706/70‑1 (MQ=255) gTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa < 1:1921482/51‑1 (MQ=255) tttATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa < 1:322736/49‑1 (MQ=255) tttATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa < 1:104034/49‑1 (MQ=255) tttATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc < 1:868032/53‑1 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa > 1:1510049/1‑49 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa < 1:181223/47‑1 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa < 1:906691/47‑1 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc > 1:778116/1‑51 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc > 1:35185/1‑51 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc > 1:175428/1‑51 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCa > 1:1097675/1‑53 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa > 1:508992/1‑65 (MQ=255) tATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCt > 1:2202546/1‑71 (MQ=255) tCTTTTTACACGCTAATCACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa > 1:2108457/1‑63 (MQ=255) tttttACACGCTAATTACTGCTGATTTTCGCGTTCAGCa > 1:267363/1‑39 (MQ=255) tttttACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCttt < 1:406746/56‑1 (MQ=255) tttACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt < 1:668799/69‑1 (MQ=255) tttACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt < 1:1135232/69‑1 (MQ=255) tttACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt < 1:981247/69‑1 (MQ=255) tttACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATAc > 1:1170090/1‑71 (MQ=255) acacGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt > 1:773204/1‑54 (MQ=255) aaTTACTGCTGATTTTCGCGTTCAGCAATAAAATCGAGCGCTTTGTTGATACGCTCGATACTGCGGGTCt > 1:29731/1‑70 (MQ=255) aaTTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATa > 1:275202/1‑51 (MQ=255) aaTTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCt > 1:1419984/1‑70 (MQ=255) | TTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCT > minE/1572247‑1572382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |