| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,581,504 | C→T | 9.1% | L577L (TTG→TTA) | ligA ← | DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,581,504 | 0 | C | T | 9.1% | 159.6 / 6.6 | 55 | L577L (TTG→TTA) | ligA | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (21/29); new base T (2/3); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAC > minE/1581435‑1581572 | aCCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGcc < 1:1510174/70‑1 (MQ=255) aaaCGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGcc < 1:1403922/64‑1 (MQ=255) cGGGCTGTCAATCTCTTCCGCGTTGATAACGATCTGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGc > 1:1491087/1‑71 (MQ=255) cGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGc > 1:1285524/1‑71 (MQ=255) cGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGc < 1:1313848/71‑1 (MQ=255) gggCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCt < 1:2069877/71‑1 (MQ=255) gTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCtgatga > 1:1387533/1‑71 (MQ=255) tCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCtgatga < 1:73935/70‑1 (MQ=255) tCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGAc < 1:1552527/71‑1 (MQ=255) tCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGAc < 1:156135/71‑1 (MQ=255) tCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGAc < 1:1635350/71‑1 (MQ=255) tCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGAc < 1:647479/71‑1 (MQ=255) tCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGAc < 1:1637980/71‑1 (MQ=255) cAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGAc < 1:181281/70‑1 (MQ=255) tctcTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACATTCCGCCAACAGCTCGCTGATGACATTg > 1:736801/1‑71 (MQ=255) ctctTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCTAACAGCTCGCTGATGACAtt > 1:675570/1‑69 (MQ=255) ctctTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCtg < 1:356189/61‑1 (MQ=255) tctTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCTAACAGCTCGCTGATGACATTGCg > 1:1099102/1‑71 (MQ=255) ccGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCtga > 1:1724355/1‑57 (MQ=255) ccGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCtga > 1:1264316/1‑57 (MQ=255) cgcgTT‑ATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGAGGTTGCt < 1:101318/71‑1 (MQ=255) gcgTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCt < 1:2204314/71‑1 (MQ=255) cgTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCt < 1:150329/70‑1 (MQ=255) gATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCTAACAGCTCGCTGATTACATTGCGGTTGCTttc < 1:1438878/69‑1 (MQ=255) gATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCTAACAGCTCGCTGATGACATTGCGGTTGCTttc < 1:1506802/69‑1 (MQ=255) gATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttctt > 1:37700/1‑71 (MQ=255) gATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttctt > 1:1102731/1‑71 (MQ=255) gATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttctt > 1:1454267/1‑71 (MQ=255) aTAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCt < 1:1217930/65‑1 (MQ=255) aTAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCt < 1:903582/65‑1 (MQ=255) tAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttc < 1:489572/67‑1 (MQ=255) tAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttc < 1:2178314/67‑1 (MQ=255) aCGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCgg > 1:272000/1‑70 (MQ=255) ggCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAaaga < 1:793588/70‑1 (MQ=255) ggCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAaaga < 1:221210/70‑1 (MQ=255) gcgcAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAaagaag < 1:1295381/71‑1 (MQ=255) gcgcAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAaagaag < 1:1900630/71‑1 (MQ=255) gcgcAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAaagaag < 1:2180791/71‑1 (MQ=255) gcgcAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAaagaag < 1:615550/71‑1 (MQ=255) aGGCGAGTGAACACCTTCCGCCTACAGCTCGCtgatga < 1:1551784/38‑1 (MQ=38) cAGTGAACACCTTCCGCTAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAAc < 1:1634699/71‑1 (MQ=255) cAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGaa > 1:324424/1‑70 (MQ=255) cAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAAc > 1:1066710/1‑71 (MQ=255) cAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAAc > 1:109269/1‑71 (MQ=255) tGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGaa > 1:1508361/1‑67 (MQ=255) tGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGaa > 1:957851/1‑67 (MQ=255) aaCACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTgg < 1:1235782/70‑1 (MQ=255) cacCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttc > 1:1845349/1‑43 (MQ=255) cacCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttc > 1:1445928/1‑43 (MQ=255) cacCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTttc > 1:1311038/1‑43 (MQ=255) acCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATg > 1:515944/1‑71 (MQ=255) ccGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAaagaatt > 1:452135/1‑49 (MQ=255) ccGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAAc < 1:461868/70‑1 (MQ=255) ccGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAAc > 1:1495995/1‑70 (MQ=255) cGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACg < 1:943648/70‑1 (MQ=255) gCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:1330319/71‑1 (MQ=255) gCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:946586/71‑1 (MQ=255) | ACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAC > minE/1581435‑1581572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |