| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 195,616 | C→T | 36.4% | L71L (CTG→CTA) | yaeF ← | predicted lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 195,616 | 0 | C | T | 36.4% | 30.5 / 30.8 | 33 | L71L (CTG→CTA) | yaeF | predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (6/15); new base T (5/7); total (11/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
ACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGA > minE/195548‑195685 | aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAg < 1:1671530/71‑1 (MQ=255) tgTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAg > 1:2146545/2‑70 (MQ=255) cGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGc < 1:2213689/71‑1 (MQ=255) tGACTCAGAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCaa < 1:1802621/71‑1 (MQ=255) tCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACa < 1:737580/63‑1 (MQ=255) acaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGcc > 1:549062/1‑71 (MQ=255) gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt < 1:1534558/71‑1 (MQ=255) gTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTgag < 1:356799/71‑1 (MQ=255) gCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt > 1:262305/1‑66 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGAt > 1:1704077/1‑70 (MQ=255) tGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga < 1:117046/66‑1 (MQ=255) gAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAg > 1:1003289/1‑71 (MQ=255) aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAgg < 1:1527036/71‑1 (MQ=255) aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCa < 1:1238006/69‑1 (MQ=255) gAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCttt > 1:2032927/1‑68 (MQ=255) aGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCgg > 1:1565297/1‑39 (MQ=255) aGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTatt < 1:474688/71‑1 (MQ=255) ggTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTatt < 1:2050221/70‑1 (MQ=255) ggTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGttt < 1:2041951/42‑1 (MQ=255) ggTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGttt < 1:2065603/42‑1 (MQ=255) gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga < 1:564712/71‑1 (MQ=255) ttACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGt < 1:2077104/38‑1 (MQ=255) ttACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGt < 1:1257988/38‑1 (MQ=255) ttACCCCAAGGCTTCAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAtt < 1:1132015/59‑1 (MQ=255) tACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAtt < 1:1311392/58‑1 (MQ=255) cAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga < 1:1794605/36‑1 (MQ=255) aGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCtt > 1:913822/1‑55 (MQ=255) ggCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCtttt < 1:1258993/56‑1 (MQ=255) ggCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCtttt < 1:1006853/56‑1 (MQ=255) gCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTg > 1:1021382/1‑70 (MQ=255) cTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTat > 1:1527137/1‑56 (MQ=255) ggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGc < 1:1644319/71‑1 (MQ=255) aCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGa > 1:883854/1‑71 (MQ=255) cAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCgg > 1:336735/1‑56 (MQ=255) | ACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGA > minE/195548‑195685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |